牡丹试管苗生根过程解剖结构观察及相关激素与酶变化的研究  

Adventitious Root Generating Process and Hormone and Enzyme Changes in Vitro Paeonia suffruticosa

作　　者：贺丹[1,2] 王政[1] 何松林[1] 

机构地区：[1]河南农业大学林学院,郑州450002 [2]北京林业大学园林学院,北京100083

出　　处：《园艺学报》2011年第4期770-776,共7页Acta Horticulturae Sinica

基　　金：国家‘863’计划项目(2002AA241131);河南省高校创新人才培养计划项目(豫教高[2005]126号)

摘　　要：以‘太平红’牡丹试管苗为材料,对其生根诱导过程中吲哚乙酸(IAA)、细胞分裂素(ZR)和脱落酸(ABA)含量,过氧化物酶(POD)、多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)活性以及根形态结构进行了研究。结果表明:试管苗不定根的根原基发生于形成层。根原基发生期为诱导生根3d左右,15d时不定根突破表皮。内源激素在根原基诱导期间整体呈下降趋势,而在不定根的伸长期整体呈上升趋势。POD活性在根原基发生期间呈下降趋势,在不定根的伸长期间波动较大;PPO和IAAO活性在根原基的诱导期间呈上升趋势,在不定根的伸长期间PPO活性先下降后上升,IAAO活性呈下降趋势。In vitro cultivar of Paeonia suffruticosa＇Taipinghong＇was used to study histological characters and the contents of IAA,ZR and ABA and the activities of POD,PPO and IAAO during rhizogenesis.Results identified that the adventitious root primordia originated from the vascular cambium cells.The adventitious root primordia formed on the 3rd day of root induction period and broke epidermis on the 15th day.The contents of endogenous hormones decreased during root primordia-inducing but increased during root elongation.Moreover,the activity of POD fell but the activity of PPO and IAAO rosed during root primordia-growing;And the activity of POD fluctuated,the activity of PPO decreased then increased,the activity of IAAO decreased during root elongation.

关 键 词：牡丹 试管苗 内源激素 酶活性 解剖结构 

分 类 号：S685.11[农业科学—观赏园艺]