不同强度电针对肥胖大鼠血脂、脂肪组织巨噬细胞趋化蛋白-1及肿瘤坏死因子-α的影响  

Effect of Different Intensities of Electroacupuncture on Expression of Monocyte Chemoattractant Protein-1 and TNF-α in Adipose Tissue in Obesity Rats

作　　者：余敏[1] 肖晓秋[2] 唐成林[3] 刘祖丽[1] 侯懿烜[1] 郜婕[1] 刘仁建[1] 

机构地区：[1]重庆医科大学中医药学院,重庆400016 [2]重庆医科大学生命科学院,重庆400016 [3]重庆医科大学科研处,重庆400016

出　　处：《针刺研究》2011年第2期79-84,共6页Acupuncture Research

基　　金：重庆市卫生局资助项目(No.07-2-63);重庆市九龙坡区科委资助项目(No.06370302)

摘　　要：目的:观察电针对单纯性肥胖大鼠脂肪组织炎性细胞因子的影响,探讨电针治疗肥胖的作用机制及不同强度电针的效应差异。方法:SD大鼠随机分为正常组、模型组、强电针(5 V)组、弱电针(2.5 V)组,每组10只。除正常组外其余各组大鼠均用高脂饲料喂养。电针组取"足三里""三阴交"穴,采用不同强度电针,每日1次,每次15 min,连续治疗14 d。观察大鼠体质量、Lee’s指数、血脂和血糖的变化,用反转录-聚合酶链反应法检测大鼠附睾脂肪组织巨噬细胞趋化蛋白-1(MCP-1)和肿瘤坏死因子-α(TNF-α)mRNA的表达。结果:模型组大鼠体质量、Lee’s指数、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、血糖(Glu)及MCP-1 mRNA、TNF-αmRNA的表达均显著高于正常组,高密度脂蛋白(HDL-C)显著低于正常组(均P<0.01);强电针组和弱电针组肥胖大鼠电针治疗后体质量、Lee’s指数、TG、TC、LDL-C、Glu及MCP-1 mRNA、TNF-αmRNA表达均明显降低(P<0.01,P<0.05),HDL-C明显升高(P<0.01,P<0.05),且强电针组优于弱电针组(P<0.05,P<0.01)。Glu含量两电针组比较,差异无统计学意义(P>0.05)。结论:电针"足三里""三阴交"穴对单纯性肥胖大鼠有一定的良性调节作用,强电针组比弱电针组效果好,其作用机制与对脂肪组织中炎性细胞因子的影响有关。Objective To observe the effect of different intensities of electroacupuncture （EA） on adipose tissue inflammatory cytokines in rats with simple obesity so as to investigate its mechanism underlying body weight reduction. Methods Forty SD male rats were randomly divided into normal, model, strong EA and weak EA groups （n = 10/group）. Obesity model was established by feeding the rats with high fat diet. EA （20 Hz, strong EA： 5 V, weak EA： 2.5 V） was applied to ＂Zusanli＂ （ST 36） and ＂Sanyinjiao＂ （SP6） for 15 min, once everyday and for 14 days. Body weight and Lee＇s index （ =body weight ~ x 10~/ body length） were detected.＂ The fasting blood glucose was detected by hexokinase method, serum triglyceride （TG） was detected by glycerol-phosphoric acid oxidase peroxydase （GPO-POD）method, total cholesterol （TO） was detected by cholesterol oxidase method, high density lipoprotein cholesterol （HDL-C） and tow density lipoprotein cholesterol （LDL-C） were measured by using one-step method, respectively. The expression of monocyte chemoattractant protein-1 （MOP-1） mRNA and tumor necrosis factor （TNF）-α mRNA in the epididymis adipose tissue was detected by reverse transcription-polymerase chain reaction（RT- PCR）. Results In comparison with the normal group, the body weight, Lee＇s index, blood lipid （TG, TO, LDL-C）, fasting blood glucose levels, and expression of MCP-1 mRNA and TNF-a mRNA were significantly higher in the model group （P〈0.01）,and HDL-C was significantly lower（ P〈0.01 ）. After EA, compared with the model group, the body weight, Lee＇s index, TG, TC, LDL-C, fasting blood glucose levels, and expression of MCP-1 mRNA and TNF-α mRNA in both strong and weak EA groups were significantly decreased（ P〈0.01, P〈0.05）, and HDL-C was significantly increased （ P〈0.01, P〈0.05）. The effects of strong EA group were obviously superior to those of weak EA group （P〈0.05, P〈0.01）. No statistical significance was observe

关 键 词：电针 肥胖 脂肪组织 炎性细胞因子 血脂 巨噬细胞趋化蛋白-1基因表达 肿瘤坏死因子-α基因表达 

分 类 号：R245.97[医药卫生—针灸推拿学]