“嗅三针”对阿尔茨海默病大鼠海马毒覃碱型受体的影响  

Effect of "Xiusanzhen" on Hippocampal Muscarinic Cholinergic Receptor Activity in Alzheimer Disease Rats

作　　者：杨晓航[1] 刘智斌[1] 牛文民[1] 牛晓梅[1] 王渊[1] 

机构地区：[1]陕西中医学院医学技术系,咸阳712046

出　　处：《针刺研究》2011年第2期90-94,共5页Acupuncture Research

基　　金：国家自然科学基金资助(No.30973792)

摘　　要：目的:探讨"嗅三针"对阿尔茨海默病(AD)模型大鼠海马胆碱能毒覃碱型受体(M受体)表达及功能的影响,了解其对嗅觉通路完整性的依赖作用。方法:成年SD雄性大鼠40只,随机分为正常对照组、AD模型组、嗅神经切断+嗅三针组和嗅三针组,每组10只。采用Aβ1-40淀粉样肽注射法制作AD大鼠模型,嗅神经切断术造大鼠嗅觉通路阻断模型,取"印堂"穴及两侧"迎香"穴交替电针刺激行"嗅三针"治疗。采用放射配体的单点结合分析法及Lowry微量定蛋白法测定海马组织胆碱能M受体最大结合量(Bmax)、平衡解离常数(Kd)及受体密度。结果:与正常对照组比较,AD模型组及嗅神经切断+嗅三针组大鼠M受体Bmax和密度显著降低(P<0.05);与AD模型组和嗅神经切断+嗅三针组比较,嗅三针组大鼠M受体Bmax和密度显著升高(P<0.05)。Kd的结果与之相反,与正常对照组比较,AD模型组及嗅神经切断+嗅三针组大鼠Kd显著提高(P<0.05);嗅三针组大鼠Kd与AD模型组和嗅神经切断+嗅三针组比较显著降低(P<0.05)。结论:"嗅三针"能够显著提高海马组织的胆碱能M受体Bmax和密度,降低Kd,其干预效应依赖于嗅觉传导通路的完整性。Objective To explore the effect of ＂Xiusanzhen＂ Eelectroacupuncture （EA） of bilateral ＂Yingxiang＂ （L120） and ＂Yintang＂ （EX-HN 3）] on activities of hipppocampal muscarinic cholinergic receptor （mAChR） and the involved neural path in Alzheimer Disease（AD）rats. Methods Forty Sprague Dawley rats were randomly divided into normal control, model,olfactory nerve severing （ONS）-EA of ＂Xiusanzhen＂ （ONS-EA） and EA of ＂Xiusanzhen＂ （EA） groups. AD model was established by intra-hippocampal injection （AP：3.5 mm, L：2 mm, H：-2.8 mm） of Aβl-40 starch-like peptide （10 μg/2 μL） under the aid of a microsyringe installed in a brain stereotaxis instrument. For rats of the ONS-EA group, the olfactory nerve was severed by using a surgical knife after drilling a hole on the skull （5 mm anterior to the bregma, L, R： 2 mm）. The mAOhR density, and its maximum binding capacity （Bmax） and dissociation constant （Kd） of the hippocampus tissue were measured by using radio-ligand binding analysis and Lowry＇s microamount protein assay. Results In comparison with the normal control group, the hippocampal mAOhR density and its Bmax in the model group were decreased remarkably（P〈0.05）, while the Kd of M-receptor in the model group was increased remarkably （P〈0.05）. In comparison with the model group, hippocampal mAOhR density and its Bmax in the EA group were up-regulated obviously（P〈0.05）, while the Kd of mAChR in the EA group was down-regulated significantly （P〈0.05）. No significant differences were found between the model and ONS-EA groups in mAOhR density and its Bmax and Kd （P〉0.05）. Conclusion ＂Xiusanzhen＇-EA can effectively up-regulate hippocampal mAOhR density and Bma. and down-regulate Kd of M-receptor of hippocampus in AD rats, which is dependent on the intact olfactory nerve pathway.

关 键 词：阿尔茨海默病 嗅三针 电针 胆碱能M受体 海马 

分 类 号：R245.97[医药卫生—针灸推拿学]