艾灸对类风湿性关节炎大鼠关节滑膜细胞超微结构的影响  

Effect of Moxibustion on Ultrastructure of Synovial Cells in Rheumatoid Arthritis Rats

作　　者：罗磊[1] 胡玲[1] 何璐[1] 唐照亮[1] 宋小鸽[1] Dirckinck-Holmfeld Lars 蔡荣林[1] 

机构地区：[1]安徽中医学院,合肥230038

出　　处：《针刺研究》2011年第2期105-109,共5页Acupuncture Research

基　　金：国家重点基础研究发展计划"973"计划(No.2009CB522905)

摘　　要：目的:观察艾灸"肾俞""足三里"穴对类风湿性关节炎模型大鼠关节滑膜细胞的影响,为艾灸抗炎的免疫作用机制提供新的依据。方法:120只雄性Wistar大鼠随机选取20只为正常组,其余大鼠采用风、寒、湿环境因素结合弗氏完全佐剂的方法复制类风湿性关节炎模型,而后随机分为模型组、艾灸组、针刺组、药物组、CO2激光组,每组20只。分别以艾灸、针刺、雷公藤灌胃、10.6μm CO2激光照射为主要治疗手段。用称量的方法得到胸腺和脾脏指数,透射电镜分析膝关节滑膜细胞内细胞器的形态学改变。结果:与正常组比较,模型组大鼠胸腺指数减小(P<0.01),脾指数增大(P<0.05);与模型组比较,艾灸可提高大鼠的胸腺指数,降低脾指数(P<0.01,P<0.05),针刺、药物、CO2激光治疗也均可提高大鼠的胸腺指数,降低脾指数(均P<0.05)。与正常组比较,模型组大鼠滑膜细胞有明显的病理改变,各治疗组均能明显改善关节滑膜细胞中的线粒体、粗面内质网等细胞器的结构。结论:艾灸不仅在整体上对胸腺、脾脏等免疫器官有保护作用,而且也能改善局部滑膜细胞的超微结构。Objective To observe the influence of moxibustion of ＂Shenshu＂（BL 23） and ＂Zusanli＂（ST 36） on the microstructure of synovial cells in the knee-joint in rheumatoid arthritis （RA） rats so as to study its underlying mechanism in anti-in- flammatory immune effect. Methods A total of 120 Wistar rats were randomized into normal control, model, acupuncture, moxibustion, CO2-1aser and medication groups （n = 20 group）. RA model was duplicated by raising the rats in a windy （electro-fan blowing）, cold [（6 ＋ 2） ℃]and wet（ 80 % - 90 % ） environment for 12 h/d and 20 days, followed by injecting Freund＇ s complete adjuvant （0. 15 mL） into the rat＇s ankle. Moxibustion or acupuncture or CO2 laser （10.6 μm） irradiation was applied to ＂Shenshu＂ （BL 23） and ＂Zusanli＂（ST 36） for 20 min, once daily for 15 days. tntragastric perfusion of Leigongteng （Tripterygium Wilfordii Hook. f. ） was given to medication group, 8 mg,kg, once daily for 15 days. The thoracic gland index [weight of the thoracic gland （mg）/body weight （g） × 100%] and spleen index [weight of the spleen （mg）/body weight （g） × 100%] were calculated after killing the rats under anesthesia. The ultrastructure of synovial cells of the knee-joint was observed by using transmission electron microscopy. Results In comparison with the normal control group, the thoracic gland index in the model group was decreased significantly （P〈0.01）, while the spleen index of the model group increased considerably （P〈0.05）. In comparison with the model group, the thoracic gland indexes in the acupuncture, moxibustion, CO2-laser and medication groups were increased significantly （ P〈0.05, P〈0. 01 ）, whereas the spleen indexes in the four groups decreased evidently （P〈0.05）. No significant differences were found among the acupuncture, moxibustion, Co2-1aser and medication groups in up-regulating the thoracic gland index and down-regulating the spleen index （ P〉0.05）. After

关 键 词：艾灸 类风湿性关节炎 滑膜细胞 超微结构 胸腺指数 脾指数 

分 类 号：R245.81[医药卫生—针灸推拿学]