硫化氢对脓毒症大鼠动脉压力反射的影响  

Effect of Hydrogen Sulfide on Arterial Baroreflex in Rat with Sepsis

作　　者：乔玮[1] 柴琛[1] 杨磊[1] 李晓云[2] 鲁彦[2] 曹农[1] 

机构地区：[1]兰州大学第一医院普外一科,甘肃兰州730000 [2]中国人民解放军第一医院检验科,甘肃兰州730030

出　　处：《中国普外基础与临床杂志》2011年第4期374-379,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助项目(项目编号:30700266);2010年兰州大学中央高校基本科研业务费自由探索项目-面上项目(自然科学类;项目编号:lzujbky-2010-146)~~

摘　　要：目的探讨硫化氢(H2S)对脓毒症大鼠动脉压力反射(ABR)的作用及机理。方法盲肠结扎穿刺(CLP)法制作脓毒症大鼠模型,选取47只雄性Spargue-Dawley大鼠随机分为9组:①假手术(SO)+0.9%NaCl(NS)静脉注射组;②SO+硫氢化钠(NaHS)静脉注射组;③CLP+NaHS静脉注射组;④SO+人工脑脊液(aCSF)双侧孤束核(NTS)注射组;⑤SO+NaHS双侧NTS注射组;⑥SO+安慰剂(DMSO)+NaHS组;⑦SO+格列苯脲(Gli)+NaHS组;⑧CLP+安慰剂(DMSO)组;⑨CLP+Gli组。分别在给药前、给药后5 min和30 min 3个时间点对各组大鼠的ABR功能进行测定。结果①同一组内不同时相间ABR值的变化结果:与给药前比较,SO+NaHS静脉注射组、CLP+NaHS静脉注射组、SO+NaHS双侧NTS注射组及SO+安慰剂+NaHS组给药后5 min和30 minABR值均明显降低(P<0.05,P<0.01),而CLP+Gli组明显升高(P<0.05)。②相同时相不同组间ABR值的变化结果:给药前,CLP+NaHS静脉注射组明显低于SO+NS静脉注射组或SO+NaHS静脉注射组(P<0.05);给药后5 min和30 min,CLP+NaHS静脉注射组明显低于SO+NS静脉注射组或SO+NaHS静脉注射组(P<0.05),SO+NaHS静脉注射组明显低于SO+NS静脉注射组(P<0.05);SO+NaHS双侧NTS注射组明显低于SO+aCSF双侧NTS注射组(P<0.01);SO+Gli+NaHS组明显高于SO+安慰剂+NaHS组(P<0.05);CLP+Gli组明显高于CLP+安慰剂组(P<0.05)。结论脓毒症H2S生成增加对ABR功能有降低作用,该作用可能与ATP敏感性钾通道开放有关;脓毒症状态下H2S不仅通过外周静脉系统发挥作用,而且可能通过中枢NTS影响ABR功能。Objective To investigate the potential role and mechanism of hydrogen sulfide （H2S） in regula- ting arterial baroreflex （ABR） in septic rats. Methods The rat model of cecal ligation and puncture （CLP） induced sepsis was established. Forty-seven male Spargue-Dawley rats were randomly divided into 9 groups：①Sham opera- tion （SO）＋0.9% NaCI（NS） intravenous injection （i. v. ） group;② SO＋NaHS i. v. group; ③ CLP＋NaHS i. v. group;④SO＋artificial cerebrospinal fluid （aCSF） bilater nucleus tractus solitarii （NTS） microinjection group;⑤ SO＋ NaHS bilater NTS mieroinjection group;⑥SO＋vehicle （DMS（））＋NaHS group;⑦SO ＋ Gli ＋ NaHS group;⑧CLP＋vehicle （DMS（）） group;⑨CLP＋GIi group. The ABR function was measured before administra tion and 5 min and 30 min after administration. Results ①The ABR value of rats at different time in the same group： Compared with the ABR value before administration in the SO＋ NaHs i.v. group, CLP＋NaHS i. v. group, SO＋NaHS bilater NTS microinjection group, and SO＋vehicle＋NaHS group, the ABR values of rats sig nificantly decreased at 5 min and 30 min after administration （P〈0.05, P〈0.01）, which significantly increased in the CLP＋GIi group at 5 min and 30 min after administration （P〈0.05）.②The ABR value of rats at the same time in the different groups： Before administration, the ABR value of rat in the CLP＋ Naris i. v. group was significantly lower than that in the SO＋NS i. v. group or SO＋NaHS i. v. group （P〈0.05）. At 5 min and 30 min after adminis-tration, the ABR value of rat in the CLP＋NaHS i. v. group was significantly lower than that in the SO＋NS i. v. group or SO＋NaHS i. v. group （P〈0.05）, which in the SO＋NaHS i. v. group or SO＋NaHS bilater NTS micro- injection group was significantly lower than that in the SO＋NS i. v. group or SO＋aCSF bilater NTS microinjection group, respectively （P〈0.05, P〈0.01 ）, in the SO＋ Gli＋NaHS group or CLPq-Gli g

关 键 词：硫化氢 脓毒症 动脉压力反射 

分 类 号：R459.7[医药卫生—急诊医学]