浆膜剥脱法建立大鼠粘连性不完全性肠梗阻模型  

Establishing Rat Model of Incomplete Adhesive Intestinal Obstruction by Serosal Stripping Method

作　　者：王东红[1] 李亮[2] 盛丽[3] 柳渊洁[4] 黄小玲[5] 王自立[6] 唐晓勇[7] 

机构地区：[1]兰州军区兰州总医院医务部,甘肃兰州730050 [2]甘肃中医学院,甘肃兰州730000 [3]甘肃省中医院超声影像科,甘肃兰州730050 [4]甘肃省中医院检验科,甘肃兰州730050 [5]甘肃省中医院病理科,甘肃兰州730050 [6]甘肃省中医院王自立工作室,甘肃兰州730050 [7]甘肃省中医院普外科,甘肃兰州730050

出　　处：《中国普外基础与临床杂志》2011年第4期384-390,共7页Chinese Journal of Bases and Clinics In General Surgery

基　　金：甘肃省自然科学研究基金计划项目(项目编号:096RJZA042)~~

摘　　要：目的探讨一种新的建立粘连性不完全性肠梗阻大鼠模型的造模方法。方法采用浆膜剥脱法建立大鼠粘连性不完全性肠梗阻模型,分别于造模后第3、5及7天各取6只大鼠取血和回肠组织,观察大鼠的一般情况变化,测量大鼠体重,行腹部彩色多普勒超声和立位X线平片检查;从股动脉取血,检测血浆D-乳酸水平;检测回肠组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;取回肠组织做病理切片,观察模型复制情况。结果与正常组(不作任何处理)比较,模型组造模后大鼠一般情况较差,体重明显下降(P<0.01);彩色多普勒超声显示肠系膜上动脉主干血流速度明显增快,小肠肠管壁明显增厚(P<0.05,P<0.01);腹部立位X线平片出现气液平面或有大量积气、积液;血浆中D-乳酸水平和回肠组织中MDA含量明显增加,到第5天时差异有统计学意义(P<0.05,P<0.01),回肠组织中SOD活性明显下降,到第7天时差异有统计学意义(P<0.05);病理观察符合粘连性肠梗阻,于第7天粘连性不完全性肠梗阻模型复制成功。结论应用浆膜剥脱法可有效建立大鼠粘连性不完全性肠梗阻模型。Objective To study a new method for establishing the rat model of incomplete adhesive intestinal obstruction. Methods The serosal stripping method was adopted, the general health condition changes of rats were observed on day 3, 5, and 7 after modeling, meanwhile the weight was measured, the superior mesenteric artery flow velocity and small intestine wall thickness were observed via the color Doppler ultrasound and orthostatic X ray. The level of D-lactate acid in the blood plasma, and the activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in the intestine tissue were tested. The pathological changes of the intestine tissue were observed. Results Compared with the normal group （no treatment was done）, the general health conditions of rats were worse, the weight significantly decreased （P〈0.01）, the superior mesenteric artery blood flow velocity signifi- cantly increased and small intestinal wall was thickened （P〈0.05, P〈0.01）, air-fluid level or a great deal of sero-peritoneum and pneumatosis flat appeared via orthostatic abdominal X-ray, the level of D-lactic acid in the bloodplasma and the content of MDA in the intestine tissue significantly increased on day 5 after modeling （P〈0.05, P〈 0.01）, the activity of SOD in the intestine tissue significantly decreased on day 7 after modeling in the model group （P〈0.05）. The pathological changes consistented with adhesive intestinal obstruction via pathological observation. On the 7th day, the rat model of incomplete adhesive intestinal obstruction was successfully built . Conclusion The rat model of incomplete adhesive intestinal obstruction is completed by serosal stripping method.

关 键 词：浆膜剥脱法 粘连性不完全性肠梗阻 模型 大鼠 

分 类 号：R656.7[医药卫生—外科学]