IL-23及其相关细胞因子在慢性特发性血小板减少性紫癜中的表达与作用探讨  

Expression and Significance of Interleukin-23 and Its Related Cytokines in Chronic Idiopathic Thrombocytopenic Purpura

作　　者：黄颖[1] 李永志[1] 魏彩霞[1] 黎承平[1] 李维佳[1] 杨弘[1] 

机构地区：[1]昆明医学院第一附属医院血液科,云南昆明650032

出　　处：《中国实验血液学杂志》2011年第2期455-458,共4页Journal of Experimental Hematology

基　　金：云南省科技厅资助项目;编号2008ZC134M

摘　　要：本研究旨在探讨lL-23及其它的IL-12家族成员在慢性特发性血小板减少性紫癜(ITP)的表达及其免疫调节功能。运用反转录实时PCR方法检测30例慢性ITP患者和15例正常对照者外周血单个核细胞(PBMNC)中IL-23p19、IL-12p35、IL-12p40、IL-27、IL-17 mRNA的表达水平,用ELISA方法检测血浆IL-23、IL-12、IL-17含量并分析其在慢性ITP中的表达规律及与T亚群的关系。结果表明,慢性ITP患者及正常人PBMNC均低水平表达IL-23p19、IL-12p35、IL-27、IL-12p40 mRNA;部分患者及正常人PBMNC微量表达IL-17 mRNA。IL-12p35、IL-27、IL-17mRNA的表达水平与正常对照相比无显著差异,IL-23p19、IL-12p40 mRNA的表达水平比正常对照明显减低(p<0.01)。慢性ITP患者血浆IL-12水平高于正常对照者(p<0.01),IL-23、IL-17水平与正常对照者相比无显著差异。结论:ITP患者体内T淋巴细胞异常主要与IL-12相关,可能与IL-23/IL-17调节轴无直接关系。The aim of this study was to investigate the expression and immunologic regulation function of interleukin-23 and its related cytokines in chronic idiopathic thrombocytopenic purpura（ITP） patients.Levels of cytokines in peripheral blood mononuclear cells（PBMNC） were detected by reverse-transcription real-time polymerase chain reaction in 30 patients with chronic ITP and 15 healthy volunteers.The quantity of IL-23,IL-12,IL-17 in serum was detected by enzyme-linked immunosorbent assay（ELISA）.The results showed that low detectable mRNA levels of IL-23p19,IL-12p35,IL-27 and IL-12p40 were found in all patients and healthy persons.Trace of IL-17 mRNA were expressed in PBMNC of part of patients and normal controls.Levels of IL-12p35,IL-27,IL-17 mRNA between healthy persons and chronic ITP patients were not statistically different.Compared with normal controls,patients showed the lower expression levels of IL-23p19 and IL-12p40 mRNA（p〈0.01）.The IL-12 levels of chronic ITP patients were significantly higher than that of normal controls（p〈0.01）.The IL-23 and IL-17 levels of chronic ITP patients were same to that of normal controls.It is concluded that the imbalance of T cell subsets in ITP patients mainly associated with IL-12,but not with IL-23 and IL-17.

关 键 词：慢性特发性血小板减少性紫癜 IL-23 IL-12 细胞因子 

分 类 号：R554.6[医药卫生—血液循环系统疾病]