阿的平对微波辐射小鼠血液系统炎症反应的影响  

Effect of Quinacrine on Inflammatory Reaction of Blood System Induced by Microwave Irradiation

作　　者：吴燕[1] 苏振涛[2] 周红梅[2] 王飞[1] 刘淑红[1] 丁学锋[1] 赵永岐[1] 范明[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国实验血液学杂志》2011年第2期499-502,共4页Journal of Experimental Hematology

基　　金：中国人民解放军总后勤部重点课题基金资助(编号01L024);国家自然科学基金课题(编号30500185)资助;中国博士后科学基金资助(编号20100480774)

摘　　要：本研究旨在探讨阿的平(Quinacrine)对微波辐射后小鼠外周血粒细胞、淋巴细胞、血清白介素1(IL-1)、白介素6(IL-6)含量的影响,观察阿的平对微波辐射引起的炎症反应的拮抗作用。130只BALB/c小鼠随机分为4组:正常对照组、辐射对照组(注射用水组)、低剂量组(阿的平12.6 mg/kg)、高剂量组(阿的平50.4 mg/kg)。小鼠灌胃给予阿的平后1小时,将辐射对照组、低剂量组、高剂量组用强度为50 mW/cm2的微波照射30分钟。在照射后即刻、1天、2天、7天处死小鼠取血,用血细胞分析仪测定外周血粒细胞数,用放射免疫法检测血清IL-1β、IL-6含量的变化。结果表明,微波辐照后粒细胞和淋巴细胞数量随时间延长明显增加,在给药组这些细胞数量改变的趋势均明显延缓;50 mW/cm2微波辐射1天后小鼠血清IL-1β水平明显升高,直至7天后方逐渐恢复至正常水平,而2个浓度的阿的平灌胃均能抑制辐射引起的血清IL-1β水平升高;50m W/cm2微波辐射后血清IL-6水平从即刻至7天逐渐升高,阿的平灌胃能够明显延缓IL-6的升高,在辐射后2天内阿的平的作用尤为明显。结论:辐照前灌胃给予阿的平能够明显延缓微波辐射引起的小鼠粒细胞和淋巴细胞数量增加,能够部分抑制血清IL-1β和IL-6水平的增高并产生了一定的拮抗作用。本研究结果提示阿的平可能有一定的抗微波辐射效应。This work was aimed to investigate the effect of quinacrine on peripheral granulocytes and lymphocytes,interleukin 1（IL-1） and interleukin 6（IL-6） in peripheral blood serum of inflammatory reaction induced by microwave irradiation,and observe the protective effect of quinacrine against microwave irradiation injury.BALB/c mice were suffered from microwave irradiation with the total intensity of 50 mW/cm2 for 30 minutes,at 1 hour before irradiation quinacrine（12.6 mg/kg or 50.4 mg/kg） was orally administrated.Mice received same volume of water for injection instead of quinacrine were named as microwave irradiation group（MR group）,and mice received no microwave irradiation but stayed in microwave irradiation environment also for 30 min were set as control.After microwave irradiation,mice were sacrificed and peripheral blood cells were analyzed with cytoanalyzer,and mice serum interleukin-1β,interleukin-6 were detected by radioimmunoassay.The results showed that microwave irradiation increased the count of peripheral granulocytes and lymphocyte along with prolongation of time,while the increase of these cells in mice administrated quinacrine was markedly delayed.The level of IL-1β in serum of mice was significantly increased after 1 day of microware irradiation（50 mW/cm2）,and recovered to normal level after 7 days.The 2 concentrations of quinacrine（12.6 mg/kg,50.4 mg/kg） could suppress level of IL-1β in serum induced by microwave irradiation.The level of IL-6 in serum of mice was gradually increased after microwave irradiation with intensity of 50 mW/cm2 for 7 days,but quinacrine administration could delay the rise of IL-6 level,specially within time of 2 days.It is concluded that the quinacrine administration can delay the increase of peripheral granulocytes and lymphocytes inducted by microwave irradiation,and may partially suppress the rise of IL-1β and IL-6 in serum.The results of this study suggest that the quinacrine can provide some protective effect aganist microwave irradiation in

关 键 词：阿的平 微波辐射 炎症反应 外周血粒细胞 外周血淋巴细胞 

分 类 号：R852.7[医药卫生—航空、航天与航海医学]