Correlation between FcγRⅢA and Aortic Atherosclerotic Plaque Destabilization in ApoE Knockout Mice and Intervention Effects of Effective Components of Chuanxiong Rhizome and Red Peony Root  

作　　者：黄烨 殷惠军 马晓娟 王景尚 刘倩 吴彩凤 陈可冀 

机构地区：[1]Department of Cardiovascular Diseases,Xiyuan Hospital,China Academy of Chinese Medical Sciences

出　　处：《Chinese Journal of Integrative Medicine》2011年第5期355-360,共6页中国结合医学杂志（英文版）

基　　金：Supported by the National Key Basic Research and Development Program of China(No.81073086)

摘　　要：Objective： To explore the correlation between Fc γ R IIIA （CD16A） and aortic atherosclerotic plaque destabilization in apoE knockout （apoE KO） mice and the intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root. Methods： Eight 8-week-old male C57BL/6J mice were selected as the control group. Forty 8-week-old male apoE KO mice were randomly divided into the model group, apoE KO ＋ intraperitoneal injection immunoglobulin group （IVIG）, apoE KO ＋ simvastatin group （Sm）, apoE KO ＋ high dosage of Xiongshao Capsule （XSC,芎芍胶囊） group （XSCH）, and apoE KO ＋ low dosage of XSC group （XSCL）, 8 mice in each group. Mice in the control group were put on a normal diet, and others were fed with a high-fat diet. After 10-week different interventions, monocyte CD16 expression was detected by flow cytometry, aortic matrix metalloproteinase-9 （MMP-9） mRNA expression was detected using reverse transcription- polymerase chain reaction, and serum tumor necrosis factor （TNF）-a level was detected using enzyme-linked immunosorbent assay. Results： Compared with the control group, monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-a level in the model group increased obviously （P〈0.01）. Injections of apoE KO mice with intraperitoneal immunoglobulin during a 5-day period significantly reduced the monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-a level （P〈0.01 or 0.05） over a 10-week period of high-fat diet. Indices above in the Sm group, XSCH group, and XSCL group decreased in a different degree. Of them, the aortic MMP-9 mRNA expression in XSCH group was lower than that in Sm group （P〈0.05） and the monocyte CD16 expression and serum TNF-a level showed no significant difference between XSCH group and Sm group （P〉0.05）. Correlation analyses suggested positive correlation between monocyte CD16 expression and aortic MMP-9 mRNA expression or serum TNF-a level in IVIG group, XSCH group, and XSCL gObjective： To explore the correlation between Fc γ R IIIA （CD16A） and aortic atherosclerotic plaque destabilization in apoE knockout （apoE KO） mice and the intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root. Methods： Eight 8-week-old male C57BL/6J mice were selected as the control group. Forty 8-week-old male apoE KO mice were randomly divided into the model group, apoE KO ＋ intraperitoneal injection immunoglobulin group （IVIG）, apoE KO ＋ simvastatin group （Sm）, apoE KO ＋ high dosage of Xiongshao Capsule （XSC,芎芍胶囊） group （XSCH）, and apoE KO ＋ low dosage of XSC group （XSCL）, 8 mice in each group. Mice in the control group were put on a normal diet, and others were fed with a high-fat diet. After 10-week different interventions, monocyte CD16 expression was detected by flow cytometry, aortic matrix metalloproteinase-9 （MMP-9） mRNA expression was detected using reverse transcription- polymerase chain reaction, and serum tumor necrosis factor （TNF）-a level was detected using enzyme-linked immunosorbent assay. Results： Compared with the control group, monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-a level in the model group increased obviously （P〈0.01）. Injections of apoE KO mice with intraperitoneal immunoglobulin during a 5-day period significantly reduced the monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-a level （P〈0.01 or 0.05） over a 10-week period of high-fat diet. Indices above in the Sm group, XSCH group, and XSCL group decreased in a different degree. Of them, the aortic MMP-9 mRNA expression in XSCH group was lower than that in Sm group （P〈0.05） and the monocyte CD16 expression and serum TNF-a level showed no significant difference between XSCH group and Sm group （P〉0.05）. Correlation analyses suggested positive correlation between monocyte CD16 expression and aortic MMP-9 mRNA expression or serum TNF-a level in IVIG group, XSCH group, and XSCL g

关 键 词：Fc γ/RIIIA apoE knockout mice matrix metalloproteinase-9 tumor necrosis factor a effectivecomponents of Chuanxiong Rhizome and Red Peony Root 

分 类 号：R285.5[医药卫生—中药学]