雷公藤多苷对糖尿病肾病大鼠足细胞病变的影响  

Effect of triperygium wilfordii polyglucoside on the podocytes of diabetic nephropathy rats

作　　者：郑云[1] 郝丽[1] 潘梦舒[1] 丁楠[1] 

机构地区：[1]安徽医科大学第二附属医院肾脏内科,合肥230601

出　　处：《中华肾脏病杂志》2011年第4期288-292,共5页Chinese Journal of Nephrology

基　　金：安徽省自然科学基金（070413066）

摘　　要：目的探讨雷公藤多苷（TWP）对糖尿病肾病大鼠足细胞病变的影响。方法SD大鼠100只，按随机数字表法分为正常对照组（A组）、糖尿病组（B组）与TWP治疗组（C组）。并将C组按4、8、16mg·kg^-1·d^-1不同给药剂量分为3组（Ca、Cb、Cc）。糖尿病组与TWP治疗组大鼠分别给予链脲菌素（STZ）一次性腹腔注射建立糖尿病大鼠模型。12周后检测24h尿蛋白量、血肌酐（Scr）、尿素氮（BUN）、外周血白细胞（WBC）、血糖（Glu）、肾质量／体质量（KW／BW）的变化；HE染色检测肾组织病理变化；透射电镜测量足细胞超微结构变化；免疫荧光法检测肾皮质nephrin和podocin蛋白表达。结果（1）与A组比较，B组及C组Scr、BUN增高（P〈0．05）；Glu、KW／BW和24h尿蛋白量显著增高（P〈0．01），除Cc组（3／20）出现肝酶（AIJT、AST）增高及WBC下降外，各组ALT、AST、WBC差异无统计学意义；肾脏皮质nephrin和podocin蛋白表达减少，肾小球、小管间质及足细胞病变明显。（2）与B组比较，C组KW／BW和24h尿蛋白量降低（P〈0．01），肾脏皮质rlephrin和podocin蛋白表达增高（均P〈0．01），肾小球、小管间质及足细胞病变明显减轻，并随雷公藤多苷剂量的增加而越加明显。结论TwP对糖尿病大鼠足细胞病变具有保护作用，并与剂量相关。其部分机制可能与上调nephrin和podocin蛋白表达有关：Objective To explore the effect of triperygium wilfordii polyglucoside （TWP） on the podocytes of rats with diabetes nephropathy （DN）. Methods One hundred SD rats were randomly divided into normal control group （Croup A）, DN group （Group B）, TWP group （Group C）. TWP group was divided into 3 subgroups （Ca, Cb, Cc） according to the different doses 4, 8, 16 mg.kg-l＇d i, respectively. Rats in DN group and TWP group were given streptozocin （STZ） by intraperitoneal injection to establish animal model of diabetes. After 12 weeks, 24 h urinary protein excretion rate（UAER）, BUN, Scr, white blood cell （WBC）, blood glucose （Glu）, and kidney weight （KW）/ body weight （BW） were determined. The renal pathological changes were evaluated by HE staining. The structural change of podocytes was observed by transmission electron microscope. The expressions of nephrin and podocin were evaluated by immunofluorescence staining. Results （1） Compared to group A, Scr, BUN, Glu, KW/BW, and UAER were significantly higher （P〈0.05） in group B and group C. Whereas the elevated liver enzymes and the decreased WBC were presented in group Cc （3/20）. Compared to group A, the protein expressions of uephrin and podocin in nephridial tissue were lower, and the significant differences of pathomorphology in glomerulus, tubules and podoeytes were observed in group B and group C. （2） Compared to group B, KW/BW and UAER were lower in group C （P〈0.01）; the protein expressions of nephrin and podocin were higher in nephridial tissue; the pathomorphologieal improvements were exhibited in glomerulus, tubules and podoeytes in group C, paralleled with the increase of TWP dose （P〈0.05 or P〈0.01）. Conclusions TWP may exert the protective effect on podoeytes in diabetic nephropathy rats, dependent on the dose of TWP. The mechanism may be associated with the up-regulatied expressions of nephrin and podocin.

关 键 词：糖尿病肾病 足细胞 雷公藤多苷 

分 类 号：R285.5[医药卫生—中药学]