复方竹叶石膏颗粒对大鼠放射性食管炎的防治作用  

Preventive and therapeutic effects of Chinese herbal medicine Compound Zhuye Shigao Granule in rats with radiation-induced esophagitis

作　　者：路军章[1] 赵丽[1] 任建平[2] 曹秀堂[3] 李海霞[4] 赵红[1] 

机构地区：[1]中国人民解放军总医院中医科,北京100853 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]中国人民解放军总医院医疗统计科,北京100853 [4]中国人民解放军总医院内科临床部,北京100853

出　　处：《中西医结合学报》2011年第4期435-441,共7页Journal of Chinese Integrative Medicine

基　　金：首都医学科技发展基金(中医药类)资助项目(No.SF-2009-Ⅲ-45)

摘　　要：目的:观察复方竹叶石膏颗粒对大鼠放射性食管炎的防治作用。方法:将56只Wistar大鼠随机分为5组:正常对照组(8只,不接受照射)、单纯照射组(12只,模型组)和复方竹叶石膏颗粒低、中、高剂量组(各12只)。分别予10mL/kg生理盐水及低、中、高剂量(1.15、2.3、4.6g/kg)的复方竹叶石膏颗粒溶液灌胃,1次/d,连续7d。采用60Co射线以490.25cGy/min剂量率一次性局部照射30Gy建立放射性食管炎模型。继续以照射前给药方法连续给药7d或14d,期间观察并记录大鼠体质量、摄食量,并于照射后第7天每组取4只大鼠,照射后第14天取每组全部存活大鼠,麻醉状态下处死取食管组织制作病理切片并观察其病理变化。结果:(1)照射后第7天,单纯照射组大鼠食管组织出现典型的放射性食管炎病理改变,而复方竹叶石膏颗粒各剂量组的放射性损伤均有不同程度的减轻,且随剂量增大损伤越轻,高剂量组食管组织基本完好。(2)高、中、低剂量组的大鼠食管组织损伤程度和炎症细胞浸润程度评分明显小于单纯照射组(P<0.05)。(3)正常对照组大鼠体质量增加而模型组大鼠体质量减少,高、中剂量组大鼠体质量未见明显减少,高剂量组大鼠体质量减少量明显小于单纯照射组(P<0.05)。(4)与正常对照组比较,其余各组大鼠照射后每日进食量均有所减少,但高、中、低剂量组大鼠进食量明显大于单纯照射组(P<0.05)。结论:复方竹叶石膏颗粒能明显减轻大鼠放射性食管炎的病理损伤程度和炎症细胞浸润,增加大鼠的进食量,防止体质量减轻,且随剂量增加效果更为显著。该结果提示复方竹叶石膏颗粒对大鼠放射性食管炎有显著的防治作用。Objective：To explore the preventive and therapeutic effects of Compound Zhuye Shigao Granule（CZSG）,a compound Chinese herbal medicine,on radiation-induced esophagitis in rats.Methods：Fifty-six Wistar rats were randomly divided into 5 groups：normal control group（8 rats）,model group（12 rats）,and high-,medium-and low-dose CZSG groups（12 rats in each group）.The rats in the normal control and model groups were given normal saline 10 mL/kg body weight and those in the CZSG-treated groups were given solution of CZSG at doses of 1.15,2.30,or 4.60 g/kg body weight respectively by intragastric administration once a day for 7 days.Then esophagitis was induced by local irradiation of 60Co ray（490.25 cGy/min,totaled 30 Gy）.The administration was continued for another 7 days or 14 days,meanwhile body weight and daily food intake of the rats were recorded.Seven days after the irradiation,4 rats in each group were sacrificed under anesthesia,then,the esophageal tissue was obtained for histopathological examination and the degrees of esophageal tissue injury and neutrophil infiltration were scored.Fourteen days after the irradiation,all the survival rats were dealt in the same way.Results：（1） Seven days after the irradiation,the esophageal tissue sections in the model group showed typical histopathological changes of radiation-induced esophagitis,whereas in the CZSG groups the histopathological changes were lessened dose-dependently and in the high-dose CZSG group the esophageal tissue remained basically intact.（2） The scores of esophageal tissue injury and cellular infiltration in the high-and medium-dose CZSG groups were both significantly less than in the model group（P0.05）.（3） The body weight of the rats increased in the normal control group,whereas it decreased obviously in the model group.In the medium-and high-dose CZSG groups,it did not decrease significantly.The decrease of body weight in the high-dose CZSG group was less than that in the model group（P0.05）.（4） Compared

关 键 词：复方 辐射损伤 实验性 食管炎 中医治法 大鼠 

分 类 号：R285.5[医药卫生—中药学]