小单孢菌叠加诱变与发酵过程的研究  

Study on Superimposed Mutation on Micromonospora purpurea and Fermentation Process in 5L Fermentor

作　　者：管玉霞[1] 孙甲贤[1] 王静[1] 郑遵纪[1] 

机构地区：[1]福州大学生物科学与工程学院,福州350002

出　　处：《中国生物工程杂志》2011年第4期77-82,共6页China Biotechnology

基　　金：<生命科学导论>精品课程建设(0360036835)资助项目

摘　　要：采用庆大霉素产生菌—绛红小单孢菌(M.purpurea)在培养36h时添加15μg/ml的青霉素,对培养至48h对数生长期的菌体进行紫外线照射+亚硝基胍复合处理,置培养箱中37℃培养5～8d待长出菌落后再用紫外线照射叠加处理,获得一高产菌株N-14。试管发酵生测效价1913u/ml(对照625u/ml);摇瓶发酵培养120h达2178(u/ml),比对照(1243u/ml)提高75.2%;并进行了5L发酵罐发酵培养,发酵过程中测定了发酵液还原糖、总糖、氨基氮、pH、菌体浓度等代谢参数,探索了适合N-14的培养条件,连续5批次生测效价平均1967u/ml,较对照1171u/ml提高了68%,效果明显。Gentamicin producing strain Micromonospora purpurea was treated with 15μg/ml penicillin when M.purpurea had cultured 36h,and the cells that had cultured for 48h at logarithmic growth phase were treated with UV and NTG,then they were cultured in incubator at 37℃ for 5～8 days,and when the colonies grew out,the cells were treated by UV again.As a result,a high yield strain N-14 was obtained.Compared to control,the biological titer of N-14 fermented in tube was increased from 625u/ml to 1913u/ml,and the biological titer of N-14 fermented in flask was 2178u/ml and increased by 75.2% compared to control 1243（u/ml）.Besides,the reducing sugar,total sugar,amino nitrogen,pH,cell concentration and other metabolic parameters were determined during fermentation process in 5L fermentor,and the culture condition was researched.The average biological titer of 5 batches was 1967u/ml and was increased by 68% compared to control 1171u/ml.

关 键 词：绛红小单孢菌 叠加诱变 生测效价 代谢参数 

分 类 号：Q813.2[生物学—生物工程]