富油能源微藻的筛选及产油性能评价  

Screening Oleaginous Microalgae and Evaluation of the Oil-producing Charateristic

作　　者：李涛[1] 李爱芬[1] 桑敏[1] 吴洪 尹顺吉 张成武[1] 

机构地区：[1]暨南大学水生生物研究中心热带亚热带水生态工程教育部工程研究中心,广州510632 [2]新奥科技发展有限公司煤基低碳能源国家重点实验室,廊坊065001

出　　处：《中国生物工程杂志》2011年第4期98-105,共8页China Biotechnology

基　　金：国家"863"计划重点项目(2009AA064401);珠海市科技局重点项目(PC20081008);培育与创新资金(暨南大学国际合作)资助项目

摘　　要：为了筛选具有产油潜力的能源微藻,以实验室保藏的20株淡水和海洋微藻(绿藻门18株,真眼点藻纲1株,硅藻纲1株)为研究对象,利用光径为3 cm柱状光生物反应器通气分批培养,通过测定微藻培养物的生物量和总脂含量等指标,从中筛选生长速度快、生物量和总脂含量高的微藻。结果表明:20株微藻的生物量和总脂含量分别在1.81～7.88g/L和16.0%～55.9%dw(%Dry weight)之间,筛选得到具有产油潜力的微藻9株,分别是栅藻(Scenedesmus sp.)(6.34g/L,55.9%dw)、麻织绿球藻(Chlorococcum tatrense)(5.93g/L,46.9%dw)、眼点拟微绿球藻(Nannochloropsis oculata)(7.88g/L,35.0%dw)、油面绿球藻(Chlorococcum oleofaciens)(5.58g/L,45.9%dw)、多形拟绿球藻(Pseudochlorococcum polymorphum)(6.10g/L,40.0%dw)、八月衣藻(Chlamydomonas augustae)(5.78g/L,40.5%dw)、椭圆小球藻(Chlorella ellipsoidea)(5.56g/L,40.7%dw)、椭圆绿球藻(Chlorococcum ellipsoideum)(5.41g/L,38.0%dw)、雪绿球藻(Chlorococcum nivale)(5.55g/L,36.3%dw),其中最具产业化潜力的微藻为栅藻(Scenedesmussp.),其总脂收获量和单位体积总脂产率分别为3.5 g/L和218.7mg/L·d。Twenty strains of freshwater and marine microalgae collected in our laboratory which belong to Chlorophytes,Eustigmatophytes and Bacillariophytes were screened for their potential of oil production.All microalgae were cultured in batch,with bubble column photobioreactor（light path,3 cm）.These microalgae were evaluated by measuring dry weight,total lipid content,total lipid yield,total lipid volumetric productivity.The results showed that the dry weight and total lipid contents of twenty microalgae were in the range of 1.81～7.88g/L and 16.0%～55.9% dw,respectively,and nine of them were referred as oleaginous microalgae,including Scenedesmus sp.（6.34g/L,55.9% dw）,Chlorococcum tatrense（5.93g/L,46.9% dw）,Nannochloropsis oculata（7.88g/L,35.0% dw）,Chlorococcum oleofaciens（5.58g/L,45.9% dw）,Pseudochlorococcum polymorphum（6.10g/L,40.0% dw）,Chlamydomonas augustae（5.78g/L,40.5% dw）,Chlorella ellipsoidea（5.56g/L,40.7% dw）,Chlorococcum ellipsoideum（5.41g/L,38.0% dw）,Chlorococcum nivale（5.55g/L,36.3% dw）.It was concluded that the most potential for industrial application was Scenedesmus sp,which lipid yield and lipid volumetric productivity was 3.5g/L and 218.7mg/L·d,respectively.

关 键 词：生物柴油 产油微藻 生物量 单位体积总脂产率 

分 类 号：Q949.93[生物学—植物学]