激素性股骨头坏死模型兔坏死股骨头内核心结合因子α1蛋白表达与普伐他汀的干预  

Interventional effect of pravastain on core binding factor alpha 1 expression in rabbit models with steroid-induced avascular necrosis of the femoral head

作　　者：胡敏[1] 范建楠[2] 赵宏斌[3] 钱传云[3] 魏蔚[3] 张扬[3] 

机构地区：[1]昆明学院医学院,云南省昆明市650032 [2]贵阳医学院附属医院骨科,贵州省贵阳市550004 [3]昆明医学院第一附属医院骨科,云南省昆明市650032

出　　处：《中国组织工程研究与临床康复》2011年第11期1933-1936,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(81060361)"恒古骨伤愈合剂对实验性骨质疏松性骨折的治疗作用及其细胞分子机制研究"及(30860389)"从 STI 及破骨相关基因表达的关系探讨彝药恒古骨伤愈合剂对骨质疏松性骨折愈合质量的影响";云南省应用基础研究面上项目(2010ZC169)"骨质疏松性骨折关键基因表达及恒古骨伤愈合剂干预研究";云南省教育厅科学研究基金项目(2010C212)"恒古骨伤愈合剂对培养的小鼠成骨细胞增殖和分化的影响";昆明市科技计划重点项目"恒古骨伤愈合剂治疗骨质疏松性骨折的临床前基础应用研"(10S090202)~~

摘　　要：背景:洛伐他汀、辛伐他汀等他汀类降脂药可上调核心结合因子α1的蛋白表达,促进骨坏死修复。目的:观察普伐他汀对激素性股骨头坏死兔模型核心结合因子α1的蛋白表达的影响。方法:将80只新西兰白兔分为对照组18只,实验组62只。实验组制备兔激素性股骨头缺血坏死模型。造模第5周,将造模成功兔36只分为模型组和他汀组,每组18只。他汀组以普伐他汀(1.2mg/kg)1次/d灌胃,模型组和对照组以等体积的蒸馏水灌胃。造模后8,12,16周分批处死动物,截取股骨头行免疫组化检测核心结合因子α1的蛋白表达。结果与结论:对照组不同时相点股骨头核心结合因子α1的蛋白表达均为阳性;模型组造模后8周及12周表达均为阴性,造模后16周表达呈弱阳性;他汀组造模后8,12,16周表达均为阳性,且随着用药时间的延长表达逐渐增强。结果说明普伐他汀可有效促进早期激素性股骨头坏死兔模型坏死股骨头核心结合因子α1的蛋白表达。BACKGROUND： Statins, such as Iovastatin and simvastatin, can up regulate expression of core binding factor al （cbfal） and promote repair of osteonecrosis. OBJECTIVE： To evaluate effect of pravastatin on protein cbfal expression in steroid-induced avascular necrosis of the femoral head （SANFH） rabbit models. METHODS： Totally 80 New Zealand white rabbits were randomly divided into a normal control group （n=18） and experimental group （n=62）. Rabbits in the experimental group were prepared for SANFH models. At 5 weeks after model preparation, 36 rabbits in the model group were assigned into the model and pravastatin groups, with 18 animals in each group. Rabbits in the pravastatin group received intragastric administration with 1.2 mg/kg pravastatin once per day, and the same volume of distilled water was given to those in the model and control groups. Rabbits in each group were killed at 8, 12 and 16 weeks respectively to evaluate of protein cbfal expression in the femoral heads using immunohistochemistry. RESULTS AND CONCLUSION： Positive protein expression of cbfal could be found in the control group at different time points; which was negative in the model group at 8 and 12 weeks, but weakly positive at 16 weeks. Expression of cbfal was positive in the pravastatin groups at 8, 12 and 16 weeks, which gradually enhanced with time prolonged. The results demonstrated that pravastatin can effectively promote protein cbfal in early SANFH rabbit models.

关 键 词：普伐他汀 核心结合因子Α1 激素性股骨头坏死 免疫组化 组织工程 

分 类 号：R318[医药卫生—生物医学工程]