烫伤创面愈合后的瘢痕形成与神经支配  

Effect of innervation on scar formation after burn wound healing

作　　者：郭鹏飞[1] 张芮 冯永强[1] 朱守荣[1] 王一兵[1] 

机构地区：[1]山东大学附属省立医院烧伤整形外科,山东省济南市250021 [2]山东医学高等专科学校病理与病理生理教研室,山东省济南市250002

出　　处：《中国组织工程研究与临床康复》2011年第11期1981-1985,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：山东省自然科学基金(Y2002C29)资助~~

摘　　要：背景：既往研究表明神经因素在创面愈合中具有重要调控作用，但有关神经调节与创面愈合后瘢痕形成之间的关系鲜有报道。目的：观察烧伤创面愈合过程中神经支配与创面愈合质量之间的关系。方法：将30只大鼠右侧T9～L1阶段脊神经根切断，制作失神经支配皮肤模型；然后在大鼠背部右侧失神经支配皮肤区域制作直径4cm的深II度烫伤创面，设为模型组，左侧对称正常皮肤制作同样的创面，设为对照组，伤后连续观察创面变化，于7，14，21d采用免疫组织化学法观察I和III胶原分泌情况，并计算I/III型胶原比例的变化，探讨创面愈合速度及愈合质量。结果与结论：模型组l，III型胶原分泌和I／HI型胶原比值于伤后各时间点均显著低于对照组（P〈0.O5）。模型组I型胶原分泌与伤后7，14，21d逐渐增3JH（P〈0.05），III型胶原于伤后21d时分泌明显增高（P〈O.05），I/III型胶原比值与伤后21d时明显降低（P〈0.05）。结果证实，创面早期神经支配可以促进创面愈合，创面重塑期减轻神经支配可能会改善创面重塑质量。BACKGROUND： Previous experiments show that neural factors play important regulating roles in wound healing, however, the relationship between neural regulation and scar formation after wound healing is known little. OBJECTIVE： To observe the relationship between innervations and wound healing in the process of scalded wound healing. METHODS： Right T9-L1 stage spinal nerve roots of 30 rats were cut off and prepared for denervation skin models; and then a diameter of 4 cm and a depth ofIIdegree scalded wound was make on the right denervated skin area of rats （model group）, the same wounds were made on the left normal skin as control group. The changes of wounds were continuously observed. The secretion of type I, Ill collagens were observed by immunohistochemical method, and type I/III collagen ratios were calculated, the speed and quality of wound healing was explored. RESULTS AND CONCLUSION： Compared with the control group, the type 1/111 collagen ratio was lower in the model group （P 〈0.05）. Secretion of type Icollagen gradually increased in the model group at 7, 14 and 21 days after injury （P〈0.05）, and type III collagen was significantly increased at 21 days after injury （P〈0.05）. Type I / IIIcollagen ratio was significantly decreased at 21 days after injury （P〈0.05）. It is revealed that early innervation of the wound may promote wound healing, and alleviating innervation on wound remodeling stage may improve the quality of wound remodeling.

关 键 词：神经支配 创面愈合 胶原蛋白 瘢痕 烫伤 组织构建 

分 类 号：R318[医药卫生—生物医学工程]