压力作用下牙周组织护骨素与Wnt-1的表达  

Expressions of osteoprotegrin and Wnt-1 in periodontal tissues under pressure force

作　　者：秦明群[1] 许连静[1] 毛峻武[1] 黄声富[2] 

机构地区：[1]桂林医学院附属医院,广西壮族自治区桂林市541001 [2]武汉大学口腔医院口腔正畸科,湖北省武汉市430079

出　　处：《中国组织工程研究与临床康复》2011年第11期2009-2012,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广西卫生厅重点科研项目(重200980)资助~~

摘　　要：背景:目前研究发现Wnt信号通路及相关因子对骨组织具有调节作用,Wnt-1与骨形成密切相关,但与正畸骨改建的相关性少有报道。目的:通过建立大鼠的正畸牙移动模型,观察压力侧牙周组织Wnt-1及护骨素的表达。方法:Wistar大鼠80只制备大鼠正畸牙移动动物模型,按牙齿移动1,3,7,14d分别取材,每只大鼠对侧牙周组织设为空白对照组。苏木精-伊红染色观察大鼠压力侧牙周组织的形态变化,然后采用RT-PCR及免疫组织化学方法检测牙周组织Wnt-1及护骨素OPG的表达。结果与结论:免疫组织化学结果与PCR检测结果显示,大鼠牙齿移动后1,3,7,14d时,压力侧护骨素均有表达,在牙齿移动后3和7d时护骨素表达明显增强(P<0.05)。Wnt-1仅在在免疫组化中检测中显示,大鼠牙齿移动后3d与对照组表达出现明显增强(P<0.05)。结果证实,在正畸力作用下,护骨素参与了正畸牙周组织改建的过程,护骨素在压力区随正畸牙齿移动表达升高具有时间依赖性。Wnt-1在正畸骨改建中的作用需进一步研究证实。BACKGROUND： Current research found Wnt signaling pathways and related factors have the adjustment function for bone tissue. Wnt-1 is closely related with bone formation, but there was no report regarding whether Wnt-1 is correlated with orthodontic bone renovation. OBJECTIVE： To investigate the changes of osteoprotegrin （OPG） and Wnt-1 expression by periodontal tissue during orthodontic tooth movement in rats. METHODS： A total of 80 male Wistar rats were prepared for orthodontic tooth movement models and drawn materials at 1, 3, 7, 14 days, respectively. Every rat＇s contralateral tooth served as control group. The morphology of periodontal tissues at pressure side were observed by hematoxylin-eosin staining; the Wnt-1 and OPG expressions at pressure side of periodontal tissue were determined by RT-PCR and immunohistochemical method. RESULTS AND CONCLUSION： RT-PCR and immunohistochemical results demonstrated that, OPG was expressed at the pressure side at 1, 3, 7, and 14 days, but the expression was enhanced at 3 and 7 days （P 〈 0.05）. Wnt-1 could be seen in the immunohistochemical results, which expressed stronger at 3 days compared with the control group （P〈0.05）. It is confirmed that in orthodontic force, OPG participates in the process of orthodontic periodontal tissue rebuilding, OPG expression increased in press area in a time-dependent manner. Correlation between Wnt-1 and orthodontic bone rebuilt need to be further researched.

关 键 词：护骨素 WNT-1 牙周组织 牙槽骨改建 组织工程 

分 类 号：R318[医药卫生—生物医学工程]