豚鼠声诱发短潜伏期负电位的来源初探  

Origin of acoustically evoked short latency negative response in guinea pigs

作　　者：黄文钦[1] 覃焕桦[1] 农东晓[1] 唐安洲[1] 李治美[1] 杨甜[1] 

机构地区：[1]广西医科大学附属第一医院耳鼻咽喉头颈外科,南宁530021

出　　处：《中华耳鼻咽喉头颈外科杂志》2011年第4期319-324,共6页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家自然科学基金（30360110）;广西科学基金（桂科回0342010）

摘　　要：目的 建立声诱发短潜伏期负电位（acoustically evoked short latency negative response,ASNR）豚鼠模型,即重度感音性聋但球囊功能正常的豚鼠,用短声刺激诱发ASNR,并通过冷热试验及前庭诱发肌源性电位（vestibular evoked myogenic potential,VEMP）来验证豚鼠的前庭功能.方法 将32只健康豚鼠按随机数字表法分为两组,对照组16只（32耳）、药物致聋组16只（32耳）.致聋组经给药（硫酸卡那霉素＋利尿酸）致聋,7～10 d后对所有动物进行听觉前庭功能检查,包括听性脑干反应（ABR）、VEMP及冷热试验.致聋组豚鼠根据ASNR的引出情况分为ASNR引出组及ASNR未引出组.结果 致聋组有11只动物（22耳）完成测试,其中8耳引出ASNR（36.4%）,阈值为120～130 dB SPL,平均（124.4±5.0）dB SPL,潜伏期l.75～2.60 ms,平均（2.15±0.27）ms,阈值平均潜伏期（2.34±0.18）ms.ASNR引出组8耳皆引出VEMP,其阈值及正负峰潜伏期与对照组比较,差异均无统计学意义（P值均＞0.05）;ASNR未引出组中有4耳引出VEMP,其阈值及正负峰潜伏期与对照组比较差异亦无统计学意义（P值均＞0.05）.ASNR引出组和未引出组VEMP引出率比较,差异具有统计学意义（P=0.002）.致聋组VEMP、ASNR的引出情况与冷热试验的眼震结果之间均无相关关系（P值均＞0.05）.结论 ASNR与反映球囊功能的VEMP具有一致性,而与代表半规管功能的冷热试验眼震结果无关,ASNR与VESP可能同起源于球囊.Objective To establish a model of acoustically evoked short latency negative response （ASNR） in guinea pigs, a model of profound hearing loss with normal saccular functions, and verify the correlation between ASNR and vestibular evoked myogenic potential （VEMP）. Methods Thirty-two healthy guinea pigs were employed in the experiment, which were randomly divided into control group （ 16subjects） and deafened group （16 subjects）. Each animal experienced auditory and vestibular tests including auditory brainstem response （ ABR）, VEMP and caloric test. A quick treatment was employed for deafened group consisting of a subcutaneous injection of kanamycin at a dose of 400 mg/kg followed by a jugular vein injection of ethacrynic acid at a dose of 40 mg/kg one hour later. The animals were received ABR, VEMP and caloric test 7 - 10 days following the drug administration. The deafened group was further divided into ASNR group and non-ASNR group, based on the presence of ASNR. Results In deafened group, five subjects died postoperatively, 11 subjects （22 ears） provided full data, ASNR was elicited in eight ears （36.4%）, the threshold was 120- 130 dB SPL with mean of （124.4 ±4.96） dB SPL. Its latency range was 1.75 - 2. 60 ms with mean of （ 2. 15 ± 0. 27 ） ms. The mean latency of threshold was （2. 34 ±0. 18） ms. All eight ASNR ears presented with VEMP. The VEMP threshold, positive and negative potential latencies proved no statistical difference （P ＞ 0. 05 ） between ASNR group and control group.Significant difference was detected between the VEMP presence of ASNR group and non-ASNR group （ P =0. 002）. There was no statistically significant correlation between VEMP and caloric test neither between ASNR and caloric test in deafened group. Conclusions This study evoked ASNR in an ototoxicity guinea pig model which has profound hearing loss with normal saccular functions. The presence of ASNR correlated with VEMP, however, not correlated with caloric test, suggesting that ASNR an

关 键 词：诱发电位 听觉 前庭诱发肌源性电位 球囊和椭圆囊 豚鼠 

分 类 号：R764[医药卫生—耳鼻咽喉科]