致儿童咽扁桃体炎酿脓链球菌emm分型  

emm typing of Streptococcus pyogenes isolated from children with adenopharyngitis

作　　者：梁云梅[1,2] 常贺生[1,3] 沈叙庄[1] 黄国英[4] 郑跃杰[5] 符洲[6] 俞桑洁[1] 袁林[1] 杨永弘[1] 

机构地区：[1]首都医科大学附属北京儿童医院,北京100045 [2]首都医科大学附属北京世纪坛医院,北京100038 [3]首都医科大学附属北京朝阳医院,北京100020 [4]复旦大学附属儿科医院,上海201102 [5]深圳市儿童医院,广东深圳518026 [6]重庆医科大学附属儿童医院,重庆400014

出　　处：《临床儿科杂志》2011年第4期382-385,共4页Journal of Clinical Pediatrics

基　　金：国家八六三计划资助项目(No.2006AA02z417)

摘　　要：目的分析导致儿童咽扁桃体炎的酿脓链球菌(Streptococcus pyogenes),即A族β溶血性链球菌(group A streptococcus,GAS)的emm分型。方法 2007年1—12月共获取119株GAS,分别来自于重庆(42株)、深圳(30株)和上海(47株)3个地区。应用多聚合酶链反应(PCR)扩增编码成熟M蛋白基因(emm基因)。对PCR扩增后的emm基因产物进行测序,并将所测得的emm基因序列提交到相关网站,获取emm分型结果。结果共有13种emm型GAS可导致儿童发生咽扁桃体炎:emm1.0(24.37%),emm3.1(0.83%),emm4.0(0.83%),emm6.5(1.68%),emm12.0(55.46%),emm22.0(10.08%),emm63.0(0.83%),emm77.0(0.83%),emm80.0(0.83%),emm102.2(1.68%),emm104.0(0.83%),st1815.0(0.83%)和stG485.0(0.83%)。重庆地区可见10种emm分型:emm1.0(11.90%),emm3.1(2.38%),emm6.5(4.76%),emm12.0(61.90%),emm22.0(4.76%),emm63.0(2.38%),emm80.0(2.38%),emm102.2(4.76%),st1815.0(2.38%)和stG485.0(2.38%)。深圳地区可见5种emm分型:emm1.0(16.67%),emm12.0(50.00%),emm22.0(26.67%),emm77.0(3.33%)和emm104.0(3.33%)。上海地区可见4种emm分型:emm1.0(40.42%),emm4.0(2.13%),emm12.0(53.19%)和emm22.0(4.26%)。结论 emm1.0、emm12.0和emm22.0克隆流行菌株是最常见的导致重庆、深圳和上海3地区儿童发生咽扁桃体炎的酿脓链球菌。3地区导致儿童发生咽扁桃体炎的克隆流行菌株存在差别。Objective To analysis the emm typing of Streptococcus pyogenes（Group A Streptococcus,GAS）isolated from children with adenopharyngitis.Methods In 2007,119 Streptococcus pyogene were isolated from children with adenopharyngitis in Chongqing（42 strains）,Shenzhen（30 strains）,and Shanghai（47 strains）.The encoding mature M protein gene（emm gene）was amplified by PCR.The amplified production of emm gene was sequenced and stored in the CDC database.The emm types of GAS strains were identified.Results Thirteen emm types,emm1.0（24.37%）,emm3.1（0.83%）,emm4.0（0.83%）,emm6.5（1.68%）,emm12.0（55.46%）,emm22.0（10.08%）,emm63.0（0.83%）,emm77.0（0.83%）,emm80.0（0.83%）,emm102.2（1.68%）,emm104.0（0.83%）,st1815.0（0.83%）,and stG485.0（0.83%）were identified in children with adenopharyngitis.Ten emm types emm1.0（11.90%）,emm3.1（2.38%）,emm6.5（4.76%）,emm12.0（61.90%）,emm22.0（4.76%）,emm63.0（2.38%）,emm80.0（2.38%）,emm102.2（4.76%）,st1815.0（2.38%）,and stG485.0（2.38%）were identified in Streptococcus pyogene isolated from Chongqing.Five emm types,emm1.0（16.67%）,emm12.0（50.00%）,emm22.0（26.67%）,emm77.0（3.33%）,and emm104.0（3.33%）were identified in Streptococcus pyogene isolated from Shenzhen.Four emm types emm1.0（40.42%）,emm4.0（2.13%）,emm12.0（53.19%）,and emm22.0（4.26%）were identified in Streptococcus pyogenes isolated from Shanghai.Conclusions The most common emm types of Streptococcus pyogene isolated from children with adenopharyngitis are emm1.0,emm12.0,and emm22.0 in Chongqing,Shenzhen,and Shanghai.The Streptococcus pyogenes isolated from children with adenopharyngitis had some differences in these three areas.

关 键 词：酿脓链球菌 咽扁桃体炎 emm基因 

分 类 号：R725.1[医药卫生—儿科]