评价大鼠铜摄入量3种指标的比较研究  

A comparison study among the three indexes of evaluating the copper intake in rats

作　　者：段链[1] 吴珍[1] 程义斌[1] 金银龙[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100020

出　　处：《癌变．畸变．突变》2011年第2期141-144,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国际铜业协会(ICA)资助项目"铜摄入量对大鼠血脂影响"(H-AS-07-02)

摘　　要：目的:筛选出对于铜摄入量敏感的指标,用于微量元素铜的健康影响评价。方法:灌胃给予大鼠不同剂量(0、0.033、0.169、0.829、4.146、20.73 mg/kg)葡萄糖酸铜,90d后测定大鼠血中铜含量、血中铜蓝蛋白活力及肝中铜含量,并进行比较研究。结果:随着铜摄入量的增加,各组大鼠血铜含量变化不明显,与正常对照组相比差异无统计学意义(P>0.05)。相关性分析表明,大鼠血中铜蓝蛋白活力随着铜摄入量的增加而上升,铜蓝蛋白活力与铜摄入量之间呈对数正相关(R^2=0.984 5,P<0.05);大鼠肝铜含量随着铜摄入量的增加呈现直线上升趋势(R^2=0.989,P<0.05)。结论:大鼠血中铜蓝蛋白活力水平及肝铜含量,均可以敏感反映出不同剂量组大鼠铜摄入水平,但血中铜蓝蛋白活力的测定方法简便,更适宜应用于大量样本的研究中评价铜摄入水平。OBJECTIVE：To obtain the sensitive index to estimate copper intake in the assessment of its impact on health.METHODS：Comparative study by determining the contents of copper in blood,and liver and ceruloplasmin（CP） activity of the rats treated by copper gluconate at different dosages（0,0.033,0.169,0.829, 4.146,20.73 mg/kg） for 90 days.RESULTS：The content of copper in blood did not change significantly in each group with the increase of copper intake,and no statistical difference compared with normal control group（P0.05）.Correlation analysis showed that CP activity rose with increasing copper intake and showed a positive logarithmic correlation （R2 =0.9845,P0.05）.The content of copper in the liver showed a linear ascending trend parallel with copper intake （R2=0.989,P0.05）.CONCLUSION：A good dose-dependent relationship was shown between the level of CP activity and copper intake,and between the content of liver copper and copper in rats.The level of CP activity and the content of liver copper in could reflect the level of copper intake at different dosage groups.Determination of CP activity is easier and the sample can be acquired easily,so is more appropriate to be used to evaluate copper intake in the research with large number samples.

关 键 词：铜 血铜含量 肝铜含量 血浆铜蓝蛋白 

分 类 号：R589.9[医药卫生—内分泌]