兔血管造影的实验研究  

Angiographic technique in rabbits:an experimental study

作　　者：陈波[1] 许健[1] 曹建民[1] 吴垠垠[1] 张龙江[1] 卢光明[1] 

机构地区：[1]南京军区总医院医学影像科,210002

出　　处：《介入放射学杂志》2011年第4期296-299,共4页Journal of Interventional Radiology

摘　　要：目的改良实验动物兔血管造影的方法,优化血管造影后的止血过程,提高DSA检查的成功率,并且对兔主要的血管的CT三维血管成像和DSA检查进行了初步研究,为兔成像的方法的顺利进行提供了相关经验。方法新西兰大白兔15只,麻醉后于耳缘静脉置管行CTA检查,测量主要血管的管径并观察相关血管形态和走行方向。解剖腹股沟区,分离股动脉和股静脉,以Seldinger法进行血管置管。动脉造影方面,穿刺股动脉后,行大动脉弓、背大动脉、腹主动脉、髂总动脉、颈总动脉、椎动脉、腹腔动脉、肠系膜前动脉和肾动脉造影,延长造影时间观察相应的回流静脉。静脉造影方面,穿刺股静脉后,依次行后大静脉和肺动脉造影。股静脉止血采用直接压迫止血数分钟即可,股动脉止血采用止血钳、血凝酶局部湿敷和外科重建股动脉鞘的方法进行。结果根据兔各主要血管平均管径的大小,选用18 G血管穿刺针和4 F导管理论上可行兔大部分血管的DSA检查。所有实验兔均成功进行了血管造影,术后成功实现了止血。结论采用18 G穿刺针和4 F导管适合进行兔主要血管的DSA检查,同时采用药物和压迫止血的方法可以成功实现术后的止血过程,避免了血管结扎引起的正常组织结构的破坏。兔血管造影的工作仍需要进一步的深入研究和完善。Objective The rabbit is the most important experimental animal for the basic interventional research,which has displayed the essential value for the interventional experimental diagnosis and therapy in some tumor and vascular disease,while the angiography is the basic imaging examination for the related study.This research attempts to improve and to optimize the angiographic technique for the experimental rabbits,as well as to modify the hemostasis course in order to increase the success rate of angiographic study in rabbits.At the same time,a preliminary study of CTA and DSA in rabbits has also been made in order to get the relative experience.Methods Fifteen New Zealand white rabbits were anesthetized,which was followed by CTA with the contrast injection through the ear vein catheterization.The diameters of the major blood vessels were measured and their shape and course were observed.An 18 G needle was used to puncture the blood vessels and a 4 F single bend catheter to perform the DSA examination.The groin area was dissected,the femoral artery and femoral vein were separated,and the catheter was inserted with Seldinger technique.For angiography examination,after the femoral artery was successfully punctured,DSA of the aortic arch,dorsal aorta,abdominal aorta,iliac artery,carotid artery,vertebral artery,celiac artery,anterior mesenteric artery and renal artery was carried out in proper sequence and the appearance of pertaining veins were also observed.For venography examination,after the femoral vein was successfully punctured,angiography of the posterior vena cava and pulmonary artery was performed successively.The femoral vein was pressed for a few minutes to stop bleeding,while the femoral artery bleeding was stanched by forceps,local wet compression with thrombin or surgical reconstruction of femoral artery sheath.Results According to the diameter and shape of the major vessels,the combination of 18G vascular puncture needle with 4 F single bend catheter could be theoretically suitable for the rabbit＇s a

关 键 词：血管造影 CT血管成像 止血 新西兰大白兔 

分 类 号：R816.2[医药卫生—放射医学]