Rapid europium-sensitized fluorescent determination of ulifloxacin,the active metabolite of prulifloxacin,in human serum and urine  

作　　者：Peng Dong Na Xu Bo Fu Lei Wang 

机构地区：[1]School of Pharmacy, Shandong University, Jinan 250012, China [2]Shandong Provincial Institute for Drug Control, Jinan 250101, China

出　　处：《Journal of Pharmaceutical Analysis》2011年第1期46-50,共5页药物分析学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.20875056,20775043)

摘　　要：A new fluorescent method was developed based on the ulifloxacin-europium（Ⅲ）-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin,the active metabolite of prulifloxacin.Sodium dodecylbenzene sulfonate formed a ternary complex with ulifloxacin-europium（Ⅲ）and significantly enhanced the characteristic fluorescence of europium（Ⅲ）.The enhanced fluorescence intensity showed a good linear relationship with the concentration of ulifloxacin in the range of 5.0×10^-8-2.0×10^-6M with a detection limit of 2.0×10^-10 M（3σ）.This method is rapid and sensitive,and has been successfully applied to the determination of ulifloxacin in human urine and serum samples.A new fluorescent method was developed based on the ulifloxacin-europium（Ⅲ）-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin,the active metabolite of prulifloxacin.Sodium dodecylbenzene sulfonate formed a ternary complex with ulifloxacin-europium（Ⅲ）and significantly enhanced the characteristic fluorescence of europium（Ⅲ）.The enhanced fluorescence intensity showed a good linear relationship with the concentration of ulifloxacin in the range of 5.0×10^-8-2.0×10^-6M with a detection limit of 2.0×10^-10 M（3σ）.This method is rapid and sensitive,and has been successfully applied to the determination of ulifloxacin in human urine and serum samples.

关 键 词：ulifloxacin PRULIFLOXACIN EUROPIUM fluorescence sodium dodecylbenzene sulfonate 

分 类 号：O557.3[理学—热学与物质分子运动论] S858.23[理学—物理]