HLA-G基因非编码区多态性与重度子痫前期的相关性  

Correlation between polymorphism of non-coding region of HLA-G gene and severe preeclampsia

作　　者：张展[1] 张楠[1] 张琳琳[1] 贾莉婷[1] 李红[1] 

机构地区：[1]郑州大学第三临床医院检验科,河南郑州450052

出　　处：《中国妇幼保健》2011年第14期2169-2172,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金项目〔30872316〕;河南省卫生厅科技攻关项目〔201002011〕;河南省科技厅科技攻关项目〔102300410005〕

摘　　要：目的:探讨HLA-G非翻译区多态性与重度子痫前期的发病有无相关性。方法:对孕晚期重度子痫前期患者、正常足月妊娠妇女,提取全血基因组DNA。利用PCR方法检测HLA-G3’UTR 14bp基因多态性,比较两组间等位基因及基因型的频率分布;利用巢氏PCR方法扩增HLA-G 5’URR上游调控区,并进行基因测序,比较两组间等位基因及基因型的频率分布。结果:①重度子痫前期组HLA-G 14bp缺失多态性的等位基因频率、基因型频率分布与正常妊娠组比较均无统计学差异(P>0.05);②HLA-G5’URR-716和-964位点基因型和等位基因型频率分布在重度子痫前期组和正常妊娠组间存在统计学差异(P<0.05);③HLA-G 5’URR-1140位点基因型和3’UTR 14bp多态性结合,在重度子痫前期组和正常妊娠组间存在统计学差异(P<0.05)。结论:①HLA-G 14bp缺失多态性的存在可减低母亲重度子痫前期的发病风险;②HLA-G 5’URR部分SNP可能与重度子痫前期的易感性相关;③HLA-G5’URR-1140位点基因型和HLA-G3’UTR 14bp多态性的结合可能与重度子痫前期的易感性有关。Objective：To explore the correlation between polymorphism of non-coding region of HLA-G gene and the occurrence of severe preeclampsia.Methods：Genomic DNA was abstracted from whole blood of normal full-term pregnant women with severe preeclampsia during the third trimester of pregnancy.PCR method was used to detect the gene polymorphism of HLA-G3＇UTR 14bp,the distributions of allele frequency and genotype frequency in the two groups were compared;Nested PCR was applied to amplify the upstream regulatory region of HLA-G 5＇URR,then gene sequencing was carried out,the distributions of allele frequency and genotype frequency in the two groups were compared.Results：There was no significant difference in distributions of allele frequency and genotype frequency of HLA-G 14bp deletion polymorphism between severe preeclampsia group and normal pregnancy group（P0.05）.There was significant difference in distributions of allele frequency and genotype frequency at HLA-G5＇URR-716 locus and-964 locus between severe preeclampsia group and normal pregnancy group（P0.05）.There was significant difference in the combination of genotype at HLA-G 5＇URR-1140 locus and polymorphism of 3＇UTR 14bp between severe preeclampsia group and normal pregnancy group（P0.05）.Conclusion：HLA-G 14bp deletion polymorphism may reduce the risk of occurrence of severe preeclampsia;partial single nucleotide polymorphism（SNP） of HLA-G 5＇URR may be related to susceptibility of severe preeclampsia;the combination of genotype at HLA-G 5＇URR-1140 locus and polymorphism of 3＇UTR 14bp may be related to susceptibility of severe preeclampsia.

关 键 词：人类白细胞抗原 重度子痫前期 HLA-G基因多态性 

分 类 号：R714.24[医药卫生—妇产科学]