加味小柴胡汤对乙型肝炎病毒转基因小鼠血清和肝组织中HBV-DNA及乙肝抗原影响的研究  

Effect of modified Xiaochaihu decoction on HBV-DNA levels in sera and liver tissue in transgenic mice

作　　者：孙晓慧[1] 王者令 刘中景[1] 

机构地区：[1]青岛市传染病医院中西医结合科,山东青岛266033

出　　处：《中国中西医结合消化杂志》2011年第2期91-93,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：青岛市中医药科研计划重点资助(2008-ZYY08)

摘　　要：[目的]观察加味小柴胡汤对乙型肝炎病毒(HBV)转基因小鼠血清和肝组织中HBV-DNA及乙肝抗原含量的影响。[方法]36只经筛选HBV-DNA阳性的转基因小鼠随机分为3组,每组12只,中药组给予加味小柴胡汤水煎剂,西药组给予拉米夫定,空白对照组给予0.85%氯化钠治疗。观察治疗后各组动物血清和肝组织中HBV-DNA含量的变化。[结果]血清HBV-DNA水平变化,与治疗前和空白组比较,中药组与西药组均有显著性改善,且两者均以西药组的改善效果显著(P<0.05,<0.01)。肝组织中HBV-DNA含量变化,与空白组进行比较,中药组对HBV-DNA含量无明显改善作用,而对HBeAg和HBsAg改善显著(P<0.05,<0.01);西药组则只对HBV-DNA有显著影响(P<0.01),对HBsAg和HBeAg的改善不显著。[结论]加味小柴胡汤能降低血清HBV-DNA水平,虽作用不及西药,但其降低HBsAg、HbeAg却明显优于西药。[Objective]To investigate the effect of modified Xiaochaihu decoction（MXD）on HBV-DNA levels in sera and liver tissue in transgenic mice.[Methods]Thirty-six transgenic mice with HBV-DNA were randomly divided into three groups：MXD group,lamivudine group and normal saline group.The HBV-DNA levels in sera and liver tissue were determined after respective treatment.[Results]The HBV-DNA levels in sera were obviously decreased after the treatment MXD or lamivudine（P0.05,P0.01）.MXD treatment improved HBsAg and HBeAg in livertissue（P0.05,P0.01）,but not HBV-DNA levels.However,lamivudine treatment decreased the levels of HBV-DNA in liver tissue,but not HBsAg and HBeAg（P0.01）.[Conclusion]MXD can decrease HBsAg and HBeAg,which is more effective than lamivudine.

关 键 词：加味小柴胡汤 乙型肝炎病毒转基因小鼠 乙型肝炎病毒-DNA 

分 类 号：R289.5[医药卫生—方剂学]