糖皮质激素对血清剥夺成人骨髓间充质干细胞及其RS细胞亚群的保护作用  

Glucocorticoid protects against serum deprivation-induced necrosis and apoptosis in a subpopulation of human marrow stromal cells

作　　者：原高明[1] 王海峰 赵勇刚[1] 史保中[1] 

机构地区：[1]河南科技大学第一附属医院神经外科,洛阳471003 [2]河南永煤集团总医院神经外科

出　　处：《陕西医学杂志》2011年第5期547-550,564,共5页Shaanxi Medical Journal

基　　金：河南省医学科技攻关资助项目

摘　　要：目的:探讨血清剥夺(SD)导致成人骨髓间充质干/祖细胞群(hMSCs)中成熟MSC(mMSC)和快速自我更新细胞(RS细胞)亚群凋亡、坏死及糖皮质激素(GC)的保护作用。方法:以无血清体外培养hMSCs为模型,经细胞形态学观察和Annexin V-PI双染流式细胞仪检测,观察细胞形态变化、核变化及活性/凋亡率变化。结果:SD 12h后hMSC皱缩变圆、染色质固缩,出现凋亡各阶段形态特征;与对照组相比mMSC及RS活性明显降低并且凋亡率升高(P<0.01),与血清剥夺组相比添加10nM DXM后细胞活性显著提高(P<0.01);并且RS细胞亚群对SD环境表现出更强的耐受性,与mMSC亚群相比存活率提高2.75倍,凋亡率降低近三分之一,并在糖皮质激素的保护作用下继续保持良好增殖。结论:糖皮质激素可以提高干细胞自我保护能力,并且RS细胞具有更强的自我保护能力和自我调节机制。Objective： To study the protective effect of glucocorticoid（GC） on mature MSC（mMSC） and rapid self-renewing cells（RS cells） in adult bone marrow mesenchymal stem / progenitor cell population（hMSCs）.Methods： By cell morphology and Annexin V-PI double staining flow cytometry the changs were observed in cell morphology,nuclear changes and the activity / apoptosis rate changes.Results：hMSC shrinkage,chromatin condensation,morphological stages of apoptosis were observed.Compared with the control group,mMSC and RS activity was significantly decreased and apoptosis increased （P0.01）;compared with serum deprived group,cell activity was significantly increased（P0.01） after adding 10nM DXM.The viability and the apoptosis percentage of cultures enriched for RS cells were 2.75-fold and 1.38-fold higher than mMSCs respectively.Conclusion： Glucocorticoid can enhance stem cell self-protection capability,and RS cells have greater capacity to self-protective and self-regulate.

关 键 词：骨髓祖代细胞/药物作用 糖皮质激素类/治疗应用 细胞凋亡 细胞计数 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学]