胶体金法检测乙肝病毒血清标志物的实验研究  

An experimental study on detection of HBV serum markers by ELISA and colloidal gold method in outpatients

作　　者：张小丽[1] 王贝晗[1] 张景[1] 张翠[1] 宋娜[1] 杨晓莉[1] 

机构地区：[1]武警总医院检验科,北京100039

出　　处：《武警医学》2011年第5期404-405,共2页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的客观评价胶体金法检测乙肝病毒标志物(HBVM)的准确性,了解胶体金法与ELISA法之间的差异。方法收集急诊手术患者的血清标本1100份,用胶体金法和ELISA法同时检测,以ELISA方法检测结果为参照,比较胶体金法检测HBVM的灵敏度和特异性。结果与ELISA胶体金法为标准计算HBsAg和HBeAg的灵敏度和特异性均超过90%;其他3个抗体的灵敏度和特异性均低于80%。结论 胶体金法检测HBsAg和HBeAg适用于急诊筛检,乙肝三项抗体金标法存在较严重的漏检,不适合急诊筛查。Objective To evaluate the accuracy of the colloidal gold method in detection of HBV serum markers. Methods A total of 1100 serum samples were examined by ELISA and colloidal gold method （GICA） , and the difference between the two methods was compared. Results According to the resuh from ELISA, the sensitivity and specificity of two antigens （ HBsAg and HBeAg） were both above 90% while the sensitivity of three antibodies was under 80%. Conculsions A test of GICA is applicable to emergency screening for both HBsAg and HBeAg, but GICA is not applicable to emergency screening for antiHBs, antiHBe and antiHBc.

关 键 词：乙型肝炎病毒 酶联免疫法 胶体金 

分 类 号：R436.1[医药卫生—临床医学]