在不同热量饮食下大鼠肾脏组织SIRT3的变化及意义  

Expression of SIRT3 in kidneys of rats on diets with different calories

作　　者：张彬[1,2] 崔敬[1] 卓莉[1] 白雪源[1] 师锁柱[1] 朱飞[1] 洪权[1] 蔡广研[1] 陈香美[1] 

机构地区：[1]解放军总医院肾内科,北京100853 [2]南开大学医学院,天津300071

出　　处：《军医进修学院学报》2011年第6期635-637,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家"973"计划基金项目(2007CB507400)~~

摘　　要：目的研究SIRT3在不同热量饮食下大鼠肾脏的表达变化规律。方法取12月龄自由饮食、热量限制、高热量饮食、健康雄性Fisher344大鼠肾组织进行衰老相关β-半乳糖苷酶(SA-β-gal)活性染色,Western印迹法(Western blot assay)和免疫组织化学法检测肾脏组织中SIRT3的表达变化与定位,并用Western blot检测p16INK4A在肾脏组织中的表达变化。结果大鼠肾脏组织SA-β-gal活性随饮食中热量的增长逐渐增强(P<0.05);p16INK4A表达随饮食中热量增加逐渐增强(P<0.05)。Western blot亦显示SIRT3蛋白表达随饮食中热量的增加逐渐减弱(P<0.05)。免疫组化结果显示,SIRT3蛋白主要表达于大鼠肾小管中,其在肾小球也有微量表达,且跟Western blot结果一致,随饮食中热量增长表达增加(P<0.05)。结论热量限制能延缓大鼠肾脏衰老,SIRT3可能与高热量引起的肾脏衰老有密切关系。Objective To study the diagnosis and treatment cost of patients with different type II diabetes mellitus（DM） subtypes and provide the reference for its control by analyzing its difference and influencing factors.Methods Data about type II DM patients admitted to a certain hospital in July 2008-February 2010 were collected.The patients were divided into different groups according to the International Classification of Diseases（ICD-10）.Hospitalization cost of the patients was statistically described with its difference and influencing factors analyzed.Results Type 2 DM was divided into 7 subtypes in 1 344 cases.The mean cost of each hospitalization time was（8.84±6.34） thousand Yuan,with a highest cost of 89.21 thousand Yuan and a lowest cost of 1.43 thousand Yuan（mean 0.72 thousand Yuan）.The fee of drugs and laboratory tests accounted for 30.99% and 30.83%,respectively.Multivariate analysis showed that type 2 DM subtypes,hospitalization time（days） and age of patients,and types of fee were the influencing factors for hospitalization cost.Conclusion The hospitalization cost is significantly different for patients with different type Ⅱ DM subtypes.Economic burden study,formulation of payment standards,control of diagnosis and treatment cost,and economic evaluation of cost should be analyzed for patients with different type Ⅱ DM subtypes.

关 键 词：SIRT3 P16INK4A SA-β-gal活性 肾脏衰老 

分 类 号：R692.9[医药卫生—泌尿科学]