乙酰唑胺对大鼠高原肺水肿的预防作用  

Preventive effect of acetazolamide on high-altitude pulmonary edema in rats

作　　者：张兆瑞[1,2] 肖华军[3] 何萍萍[2] 王桂友[3] 邓昌磊[3] 顾昭[3] 王东[2] 张波[2] 

机构地区：[1]解放军总医院军医进修学院,北京100853 [2]空军总医院呼吸内科,北京100142 [3]航空医学研究所,北京100142

出　　处：《军医进修学院学报》2011年第6期648-650,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的探讨乙酰唑胺对大鼠高原肺水肿的预防作用及其机制。方法雄性Wistar大鼠随机分为3组,分别为对照组、安慰剂组、乙酰唑胺组。建立大鼠高原肺水肿模型,观察乙酰唑胺100mg/(kg.d)干预5d后大鼠的肺湿干比(W/D),肺泡灌洗液(BALF)蛋白浓度,肺损伤病理积分,血清和肺组织中一氧化氮(NO)含量、一氧化氮合酶(NOS)活力的变化。结果安慰剂组大鼠肺W/D、BALF蛋白浓度、肺损伤病理积分明显高于对照组(P<0.05),乙酰唑胺组肺湿干比、BALF蛋白浓度、肺水肿及出血积分较安慰剂组显著降低(P<0.05)。安慰剂组大鼠血清及肺组织匀浆中NO含量及NOS活力显著低于对照组,乙酰唑胺组大鼠血清及肺组织匀浆中NO含量及NOS活力显著高于安慰剂组(P<0.05)。结论乙酰唑胺给药5d可有效预防大鼠高原肺水肿,其作用机制可能与上调血清及肺组织内NO含量及NOS活力有关。Objective To study the preventive effect of acetazolamide（AZ） on high-altitude pulmonary edema（HAPE） in rats and its possible mechanism.Methods Male Wistar rats were randomly divided into control group,AZ group,and placebo group.A rat HAPE model was established.Five days after the rats were interfered with AZ at the dose of 100mg/（kg·d）,the wet/ dry（W/D） ratio,protein concentration in bronchoalveolar lavage fluid（BALF）,pulmonary edema and hemorrhage score,nitric oxide（NO） level and NO synthase（NOS） activity in serum and lung tissue of rats were measured.Results The W/D ratio,protein concentration of BALF,pulmonary edema and hemorrhage score were significantly higher in placebo group than in control group（P〈0.05） and significantly lower in AZ group than in placebo group（P〈0.05）.The NO level and NOS activity in serum and lung tissue were significantly lower in placebo group than in control group and significantly higher in AZ group than in placebo group（P〈0.05）.Conclusion Administration of AZ for 5d can effectively prevent HAPE in rats by up-regulating NO level and NOS activity in serum and lung tissue.

关 键 词：高原肺水肿 大鼠 乙酰唑胺 一氧化氮 一氧化氮合酶 

分 类 号：R135.6[医药卫生—劳动卫生]