橙皮苷的高效液相色谱定量分析  

Study on quantitative determination of hesperidin by high performance liquid chromatography

作　　者：宁方刚[1] 许建中[2] 李小波[3] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,厦门361005 [2]国家海洋局第三海洋研究所,厦门361005 [3]厦门大学化学化工学院化学实验教学中心,厦门361005

出　　处：《中国食品添加剂》2011年第2期220-223,234,共5页China Food Additives

基　　金：福建省科技计划重点项目(2010Y0035)

摘　　要：目的:建立快速灵敏检测橙皮苷的高效液相色谱法(HPLC)。方法:采用Hypersil ODS2色谱柱,以乙腈-水-冰醋酸为流动相来检测橙皮苷含量。结果:橙皮苷溶液在3.00～30.00mg/L范围内浓度与峰面积线性关系良好,相关系数r≥0.9997;方法的检测限为6.00ng;精密度实验表明连续6次进样橙皮苷峰面积的相对标准偏差(RSD)为0.08%;加标回收率在98.80%～103.12%。稳定性实验表明在4℃下放置8天,橙皮苷的降解率均小于1.70%;在30℃下放置8天,橙皮苷的降解率均小于1.59%。结论:本方法简单、可靠、灵敏,可用于橙皮苷的精密定量分析。Objective：To develop and validate a sensitive and specific high performance liquid chromatography（HPLC）method for the determination of hesperidin.Method：Hesperidin was analyzed by a Hypersil ODS2 column using 16 ∶84（v/v）acetonitrile/water as eluent which containede 0.06% acetic acid.Results：The method for analyzing hesperidin was proved to be accurate and precise with the linearity range being of 3.00 to 30.00mg/L.The linear correlation coefficient r≥0.9997 and the limitation of the detection for the method was 6.00ng.The precision experiment,carried out by injecting the same hesperidin sample for six times,showed that the relative standard deviation（RSD）for the peak areas was 0.08%.The recovery rate was 98.80%～103.12%.The stability experiment indicated that the degradation rates of hesperidin were all less than 1.70% and 1.59% within 8 days when stored at 4℃ and 30℃,respectively.Conclusion：The method established was simple,accurate and sensitive.It is suitable to analyze hesperidin using by HPLC method.

关 键 词：高效液相色谱法 橙皮苷 检测限 精密度 加标回收率 稳定性 

分 类 号：O657.7[理学—分析化学]