Ginsenoside Rg_1 reduced the invasion of S.aureus into respiratory epithelial cells involving pro-inflammatory cytokines and glucocorticoid receptor  

人参皂苷Rg_1通过作用于炎性因子和糖皮质激素受体减少金葡菌对肺上皮细胞的损伤(英文)

作　　者：袁梽漪[1,2] 孟甄[1,2] 柴玉爽[1,2] 兰嘉琦 雷帆 李慧颖[1,2] 邢东明[1,2] 李慧玉 杜力军[1,2] 

机构地区：[1]清华大学教育部蛋白质科学重点实验室 [2]清华大学医学院生命科学学院药物药理研究室,北京100084

出　　处：《Journal of Chinese Pharmaceutical Sciences》2011年第3期266-274,共9页中国药学（英文版）

基　　金：National Natural Science Foundation of China (Grant No.30973896,30801523 and 81073092);the National S&T Major Special Project for New Drug R&D of China(Grant No. 2009ZX09103 -301,2009ZX09502 and 2011ZX09101-002-11 )

摘　　要：The invasion of Staphylococcus aureus into respiratory epithelial cells and the followed inflammatory responses cause serious tissue damage.The aim of this study was to investigate the effects of ginsenoside Rg_1（Rg_1）on S.aureus infection in vitro and its action mechanism.An internalization model was constructed to determine the effect of Rg_1 on S.aureus invasion. The changes of expression of integrinβ1,NF-κB and glucocorticoid receptor were analyzed by Western blot.Expression of pro-inflammatory genes was validated using RT-PCR.The results demonstrated that Rg_1 treatment could reduce the invasion of S.aureus into rat pulmonary epithelial cells by down-regulating integrinβ1.Its anti-inflammatory action was exerted through reducing NF-κB and expressions of intercellular adhesion molecule-1（ICAM-1）,tumor necrosis factor-α（TNF-α）,interleukin-2 （IL-2）and interleukin-6（IL-6）.The increased expression of glucocorticoid receptor was involved in this regulation.The results suggested that Rg_1 could play a positive role in reducing S.aureus infections.Rg_1 could be used for the treatment of S.aureus infection,potentially.本文旨在探讨人参皂苷Rg_1体外对金葡菌感染肺上皮细胞所致病变的作用及其可能的机制。实验采用体外大鼠原代肺上皮细胞感染模型。RT-PCR和Western blot方法观察肺细胞中integrinβ1,NF-κB和糖皮质激素受体(GR)等相关因子的表达。结果表明人参皂苷Rg_1可以明显抑制金葡菌感染后肺上皮细胞整合素integrinβ1的表达,下调炎性因子NF-κB,ICAM-1,TNF-α,IL-2和IL-6,上调GR的表达。人参皂苷Rg_1可以明显抑制金葡菌感染肺上皮细胞后的炎性因子,从而对肺脏起到一定的保护作用。

关 键 词：Staphylococcus aureus Ginsenoside Rg_1 Pulmonary epithelial cell 

分 类 号：R285.5[医药卫生—中药学]