pH值对纯菌种Ethanologenbacterium sp.nov R3产氢的影响  

Effects of pH value on fermentative biohydrogen production with Ethanologenbacterium sp.nov R3 in continous stirred tank reactor

作　　者：史沫男[1] 林永波[1] 邓洁璇[1] 陈红[1] 李永峰[1,2] 

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]上海工程技术大学化学化工学院,上海201620

出　　处：《哈尔滨商业大学学报（自然科学版）》2011年第2期165-168,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家863项目(2006AA05Z109);上海市重点科技攻关项目(071605122)

摘　　要：以Ethanologenbacterium sp.nov R3(以下称为R3)为研究对象,将其接种入连续搅拌槽式反应器(continuous stirred tank reactor,CSTR)中进行发酵产氢实验,以葡萄饮料废水为底物,温度控制在(35±1)℃,水力停留时间为8 h,探讨了不同的进水pH值对纯菌种R3连续流厌氧发酵生物制氢的影响.实验证明,当进水pH值为5.5时R3的厌氧发酵产氢能力最强.此时,产气量和氢气体积分数分别达到了6.85～8.86 L/d和59.44%～65.13%,COD去除率稳定在26%左右,出水pH值稳定在4.38左右.在总挥发酸中,乙醇和乙酸的平均质量浓度分别是706 mg/L和446 mg/L.整个过程中,R3始终保持乙醇型发酵特性.In this paper,Ethanologenbacterium sp.nov R3 was used as research microbe which was inoculated from continuous stirred tank reactor（CSTR）.The temperature and HRT of CSTR were controlled at（35±1）℃ and 8 h respectively.The effects of influent pH value on fermentative biohydrogen production with R3 were showed in this paper.When influent pH value was 5.5,the capacity of R3 to produce hydrogen was best.In this influent pH value,biogas yield and hydrogen content respectively achieved 6.85～8.86 L/d and 59.44%～65.13%.COD removal and effluent pH value were mainly stable at 26% and 4.38.The average concentration of ethanol and acetic acid in VFAs were respectively 706 mg/L and 446 mg/L.R3 maintained ethanol-type fermentation characteristics all along.

关 键 词：Ethanologenbacterium SP.NOV R3 进水pH值 生物制氢 CSTR 

分 类 号：Q939.9[生物学—微生物学]