丝胶对糖尿病肾病大鼠肾血管内皮生长因子和色素上皮衍生因子表达的影响  

Effects of sericine on vascular endothelial growth factor and pigment epithelium-derived factor expression il/the kidney of diabetic nephropathy rat

作　　者：宋成军[1] 李健[2] 郝祥俊[1] 付秀美[1] 陈志宏[1] 

机构地区：[1]承德医学院,承德067000 [2]承德市中心医院,承德067000

出　　处：《解剖学杂志》2011年第2期183-187,共5页Chinese Journal of Anatomy

基　　金：河北省教育厅资助项目（2006301）;河北省科技厅资助项目（08276101D19）

摘　　要：目的：观察彩色蚕茧提取物-丝胶对糖尿病肾病大鼠肾血管内皮生长因子（VEGF）和色素上皮衍生因子（PEDF）表达的影响.方法：雄性SD大鼠随机分为正常对照组、糖尿病肾病模型组、丝胶治疗组、二甲双胍治疗组和丝胶预防组.建立链脲佐菌素致动物模型,以血糖≥16.7mmol/L作为成模标准;丝胶治疗组和二甲双胍组大鼠分别给予丝胶（2.4g·kg-1·d-1,35d）和二甲双胍（55.33mg·kg-1·d-1,35d）灌胃;丝胶预防组大鼠于注射链脲佐菌素前给予丝胶（2.4g·kg-1·d-1）灌胃35d.分别检测各组大鼠的血糖和24h尿蛋白;免疫组织化学显色和免疫印迹法观察肾VEGF和PEDF蛋白的表达.结果：与正常对照组大鼠相比,模型组大鼠的血糖、24h尿蛋白定量和肾VEGF蛋白的表达升高,肾PEDF蛋白的表达降低;丝胶治疗组、丝胶预防组和二甲双胍组大鼠的血糖、24h尿蛋白定量和肾VEGF蛋白的表达低于模型组,肾PEDF蛋白的表达高于模型组,且丝胶治疗组、丝胶预防组与二甲双胍组比较无差别.结论：丝胶可通过上调肾PEDF蛋白、下调肾VEGF蛋白改善糖尿病肾病时肾血管生成的不平衡,发挥对糖尿病肾病大鼠肾的保护和预防作用.Objective： To observe the effects of color silk cocoon extraetion-serieine on vascular endothelial growth factor （VEGF） and pigment epithelium-derived factor （PEDF） expression in the kidney of diabetic nephropathy （DN） rats. Methods： 60 male SD rats were randomly divided into 5 groups （n = 12） ： normal control group, DN model group, sericine treatment group, metformin treatment group and sericine prevention group. DN rats models were established in the model group, sericine treatment group, metformin treatment group and sericine prevention group by intraperitoneally injected streptozotocin and blood glucose≥16.7 mmol/L was taken as standard. After the rats model were successfully established： the rats in the sericine treatment group and metformin treatment group were respectively lavaged with sericine （2. 4 g · kg-1· d-1 , 35 d） and mefformin （55.33 mg· kg-1 · d-1· 35 d） ; the rats in the sericine prevention group were lavaged with the same dose sericine （2.4 g · kg 1 · d-1 ） for 35 d before injecting streptozotocin. The blood glucose and 24- hour urine protein of rats in each group was detected, hnmunohistostaining and Western blotting were used to detect the expression of VEGF and PEDF in the kidney. Results： Compared with the normal control rats： the blood glucose, 24-hour urine protein and VEGF expression in the kidney of rats in the model group increased obviously; PEDF expression in the kidney decreased obviously. The blood glucose, 24-hour urine protein and VEGF expressions in the kidney of rats in the sericine treatment group, sericine prevention group and mefformin treatment group were significantly lower than those of the model group; PEDF expression in the kidney was significantly higher. Moreover, there were no obvious differences between the sericine treatment group, sericine prevention group and metformin treatment group. Conclusion： Sericine can improve imbalance of vascularization in the kidney of DN rats by up-regulating PEDF and down-regulating

关 键 词：丝胶 糖尿病肾病 肾 血管内皮生长因子 色素上皮衍生因子 

分 类 号：R285.5[医药卫生—中药学]