龟板抑制帕金森病大鼠T淋巴细胞的浸润  

Plastrum testudinis inhibits the infiltration of T lymphocytes in Parkinson＇s disease

作　　者：伍艺灵[1] 刘洋[1] 曹佳会[1] 陈东风[1] 周健洪[1] 李伊为[1] 黎辉[1] 邓汝东[1] 张赛霞[1] 

机构地区：[1]广州中医药大学解剖学教研室,广州510405

出　　处：《解剖学杂志》2011年第2期191-194,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（30772861）

摘　　要：目的：观察龟板是否能抑制CD3＋、CD4＋、CD8＋T淋巴细胞的浸润和趋化因子受体CCR3的表达.方法：本实验用6-羟基多巴胺（6-OHDA,0.2%）于大鼠左侧黑质致密带注射2μl造成帕金森病模型,设立正常对照组、模型组和龟板组,应用免疫组织化学显色方法观察帕金森病大鼠中脑黑质中CD3＋、CD4＋、CD8＋淋巴细胞和CCR3细胞阳性细胞数目.结果：免疫组织化学显色显示正常对照组没有检测出CD3＋、CD4＋、CD8＋、CCR3阳性细胞,模型组帕金森病大鼠中脑黑质的CD3＋、CD4＋、CD8＋、CCR3阳性细胞数增多,龟板组帕金森病大鼠中脑黑质的CD3＋、CD4＋、CD8＋、CCR3阳性细胞数少于模型组.结论：龟板能抑制CD3＋、CD4＋、CD8＋淋巴细胞的浸润和趋化因子受体CCR3的表达.Objective： To observe whether plastrum testudinis （PT） can inhibit the infiltration of T lymphocytes and the expression of chemokines receptor or not. Methods： 6-OHDA （0.2 %, 2μl） was injected into the rat＇s compact zone of substantia nigra of the left side to induce Parkinson＇s disease （PD） in the present study. All animals were randomly divided into three groups： normal group, model group and PT treatment group. The number of CD3＋ , CD4＋ and CD8＋ T lymphocytes positive cells and CCR3 positive cells in suhstantia nigra was measured by immunohistochemistry. Results： There were no positive cells in the normal group. The number of CD3＋ , CD4＋ and CD8＋ positive T lymphocytes and CCR3 positive cells in substantia nigra were increased in the model group, and decreased in the PT treatment group. Conclusion： These results indicate that PT can inhibit the infiltration of T lymphocytes and the expression of chemokines receptor of PD rats.

关 键 词：龟板 帕金森病 免疫炎症 CD3+ CD4+ CD8+ CCR3 

分 类 号：R392.6[医药卫生—免疫学]