核因子-κB在糖尿病性阴茎勃起功能障碍中的作用  

Effect of NF-KB on diabetic erectile dysfunction

作　　者：刘丽[2] 王文进[1] 朱浩[1] 李志宏[1] 丁文龙[1] 陈明峰[1] 

机构地区：[1]上海交通大学医学院解剖学教研室,上海200025 [2]上海健康职业技术学院解剖学教研室,上海200237

出　　处：《解剖学杂志》2011年第2期203-206,共4页Chinese Journal of Anatomy

基　　金：上海市自然科学基金（03ZRl4042）;上海市重点学科建设项目（S30201）

摘　　要：目的：探讨核因子-κB（NF-κB）在糖尿病（DM）性阴茎勃起功能障碍（ED）中的作用.方法：注射链脲佐菌素建立糖尿病大鼠模型,分别在注射8周和12周后观察阴茎勃起次数、取大鼠阴茎和脊髓第2～4骶椎节段,免疫印迹法检测阴茎核因子-κB（NF-κB）蛋白量的变化,ABC免疫组织化学方法检测脊髓第2～4骶髓节段NF-κB的分布和表达.结果：糖尿病大鼠的阴茎勃起次数明显低于对照组,并随病程延长降低;免疫印迹法结果显示,糖尿病组阴茎NF-κB蛋白量明显升高,12周糖尿病组与8周糖尿病组比较升高明显;NF-κB在脊髓主要分布于脊髓前角的大型运动神经元,12周糖尿病组分别与对照组和8周糖尿病组比较核转移率升高.结论：糖尿病大鼠阴茎NF-κB蛋白量的升高、脊髓第2～4骶髓节段前角NF-κB表达的升高及活性的增强可能参与了糖尿病性ED的形成.Objective： To investigate the effect of NF-kB on diabetic erectile dysfunction （ED）. Methods： STZ was injected to induce diabetes mellitus （DM）. Eight and 12 weeks later, penis erectile function was detected by injecting apomorphine. The penile shaft and S2-4 of the spinal cord were excised from rats, and Western blotting was carried out on the control and diabetic penis samples to examine the change of NF-kB. NF-kB expression in S2-4 of the spinal cord was examined by immunohistochemisty technique. Results： Compared with the control group, the erection duration of DM rats was significantly decreased （P〈0.01）, and the expression of NF-kB in the penis was increased. The rate of nuclear transloeation of NF-kB in the spinal cordS2-4 in 12 weeks DM group was higher than that in the control group （P〈0.05）. Conclusion： Increased expression of NF-kB are probably involved in the mechanism of diabetic ED.

关 键 词：糖尿病 阴茎 勃起功能障碍 脊髓 核因子-KB 

分 类 号：R977.1[医药卫生—药品]