西藏藏族人群白细胞介素1受体拮抗剂基因多态性  

Polymorphism of interleukin-1 receptor antagonist gene in Tibetan population

作　　者：李宁[1] 任甫[2] 宋慧娟[1] 苏荣健[1] 温有峰[1] 席焕久[2] 

机构地区：[1]辽宁医学院基础学院,锦州121000 [2]辽宁医学院生物人类学研究所,锦州121000

出　　处：《解剖学杂志》2011年第2期257-259,共3页Chinese Journal of Anatomy

基　　金：国家自然科学基金（30971529）;辽宁省教育厅基金（L2010254）

摘　　要：目的：研究白细胞介素-1受体拮抗剂（IL-1Ra）在西藏藏族健康人群中的分布特点,并与其他不同人群进行比较.方法：采用PCR的方法,对125名西藏拉萨市藏族人群IL-1Ra基因的可变数目串联重复序列多态性进行检测,计算其基因型频率和等位基因频率,并结合文献与其他不同人群进行比较分析.结果：西藏藏族人群IL-1Ra位点基因型以A1/A1纯合子型最为多见（频率为90.40%）,A1/A2杂合子型次之（频率为9.60%）,A2/A2纯合子型未检测到;其等位基因分布也是以A1等位基因最为多见 （频率为95.20%）,其次为A2等位基因（频率为4.80%）.西藏藏族人群的等位基因频率分布与美国人、德国人、非洲白人差异较大,具有统计学意义.而与亚洲人群包括日本人和中国汉族差异较小.结论：西藏拉萨市藏族人群中IL-1Ra位点以A1等位基因为主,其多态性分布与其他人群之间存在明显的差异,为进一步研究IL-1Ra基因多态性与疾病的关系奠定了基础.Objective： To study genotype and allele distribution of interleukin-1 receptor antagonist （IL-1Ra） in Tibetan population, and to compare and analyze the distribution of SNP among different races. Methods： Using polymerase chain reaction method, the polymorphism of IL-1Ra in 125 Tibetan population was detected, and the genotype and allele frequencies were calculated and analyzed. Results： In Tibetan population the most frequent genotype was A1/A1 （90.40%）, and the second was A1/A2（9.60%） ; the most frequent allele was A1（95.20%）, and the second was A2（4.80%）. There was significant difference in the frequency distribution of IL-1Ra allele between American, Germany and White African, while no difference in the polymorphism of IL-Ra with Chinese Han and Japanese. Conclusion： In Tibetan population, the most frequency allele was A1 of IL-Ra. For the SNP distribution of IL-Ra, there was difference between different population, which may be of significance in the studies on the relationship between genetics and disease association.

关 键 词：白细胞介素-1 多态性 聚合酶链反应-限制性片段长度多态性 

分 类 号：R392.11[医药卫生—免疫学]