急性缺血心肌无针喷射双生长因子藻酸盐水凝胶的孔道效应  

Channel Effects of Needleless Jetting Alginate Hydrogels with Two Growth Factors

作　　者：周志益[1] 任建丽[2] 吴奇[1] 黄晶[1] 王志刚[2] 

机构地区：[1]重庆医科大学附属第二医院心内科,重庆市400010 [2]重庆医科大学超声影像学研究所

出　　处：《中国超声医学杂志》2011年第4期297-300,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金专项基金项目(No.30527001);国家自然科学基金青年科学基金项目(No.81000621);重庆市科技攻关计划项目(No.CSTS2009AB5003)

摘　　要：目的采用无针喷射心肌打孔,观察无针喷射生长因子藻酸盐水凝胶的孔道效应。方法制备包裹血管内皮细胞生长因子和血小板源性生长因子藻酸盐水凝胶。成功建立兔心肌梗死模型后,随机分成3组:盐水组、凝胶组和生长因子凝胶组,随后行无针喷射心肌打孔,术中采用超声诊断仪监控无针喷射过程。术后8周.心脏超声造影观察孔道效应,造影后2d进行HE染色及Masson染色。结果肉眼观察可见白色糊状凝胶填充于孔道内部。超声诊断仪可全程监控无针喷射过程。心脏超声造影结果显示生长因子凝胶组超声微泡造影剂可沿孔道直接灌注心肌,其余两组未见造影剂经心室腔直接灌注心肌。HE染色结果显示生长因子凝胶组在心肌梗死周边无针喷射区可观察到孔道形成,其余两组孔道已闭合。Masson染色结果显示生长因子凝胶组孔道外壁包绕较多染有蓝色的胶原纤维,而孔道维持开放,其余两组孔道被染有蓝色的胶原纤维填充。结论无针喷射包裹生长因子藻酸盐水凝胶可于心肌梗死周边区建立孔道,实现血液经心室腔沿孔道直接灌注缺血心肌。Objective To investigate channel effects of needleless jetting alginate hydrogels with two growth factors. Methods Alginate hydrogel incorporated with two growth factors（vascular endothelial growth factor and platelet-derived growth factor） were prepared. 22 rabbits were randomly assigned into three groups after myocardial infarction models Were prepared..A, lginate hydrogels incorporated with two growth factors were given to gel ＋ growth factors group （n=8）. Alginate hydrogels were given to gel group（n=8）. Normal saline was given to saline group （n= 6）. Ultrasound diagnostic equipment was used to monitor the progress of needless jetting. Eight weeks after procedure, contrast echocardiography was used to detect patent channel. Myocardial histological sections were stained with HE stain and Masson stain. Results White pasty gels were viewed in channels. Jetting progress was monitored by ultrasound diagnostic equipment. Contrast echocardiography demonstrated that patent channels in gel＋ growth factors group were perfused by ultrasound contrast agent. There were no ultrasound contrast agent perfusing in the other two groups. HE stain showed patent channels were observed in the gel＋growth factors group, while no patent channels were observed in the other two groups. Masson stain showed there was much blue collagen fiber around the channels in gel＋growth factors group and much lumen was left in the channels, while the channels in the other two groups had been filled by fibers. Conclusions Needleless jetting alginate hydrogel incorporated with two growth factors could induce patent channels. Ishemic myocardium can be perfused directly by intraventricular oxygenated blood through pa- tent channels.

关 键 词：无针喷射 生长因子 藻酸盐水凝胶 孔道 

分 类 号：R542.2[医药卫生—心血管疾病] R445.1[医药卫生—内科学]