辐射诱导小鼠外周血网织红细胞微核与骨髓嗜多染红细胞微核的剂量-效应关系  

Correlation analysis on MN-RET from peripheral blood and MN-PCE from bone marrow in micefollowing exposure to irradiation

作　　者：刘丽波[1] 张俭[2] 刘宇光[3] 倪冠英[2] 刘树铮[1] 

机构地区：[1]吉林大学公共卫生学院,长春130021 [2]吉林省肿瘤医院 [3]北京市海淀区疾病预防控制中心

出　　处：《中华放射医学与防护杂志》2011年第2期122-125,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的观察x射线照射后小鼠外周血网织红细胞微核（MN-RET）和骨髓嗜多染红细胞微核（MN-PCE）的变化，为探索快速、高通量的辐射生物剂量计奠定基础。方法54只ICR雄性小鼠随机分为3组，每组18只，分别施予x射线全身照射，吸收剂量分别为0、0．5、1、2、4和5Gy。在照射后24、48和72h，分别采用流式细胞术（FCM）和人工镜检的方法，观察外周血MN-RET和骨髓MN-PCE的变化。结果在0.5-5.0Gy范围内，3个时间点外周血MN-RET和骨髓MN-PCE均随剂量的增加而升高，剂量-效应曲线可拟合成直线回归方程（t=10.26-25．77，P〈0.05）；外周血MN-RET和骨髓MN·PCE之间明显相关（r=0.986-0.996，P〈0.05）。结论FCM检测MN-RET有望成为快速、高通量的辐射生物剂量计或辅助诊断方法。Objective To study the changes of reticulocyte micronucleus （MN-RET） from peripheral blood and polychromatic erythrocyte micronucleus （MN-PCE） from bone marrow in mice following exposure to X-rays in order to provide an experimental basis for exploring possible high-throughput radiation biodosimeter. Methods Male ICR mice were whole-body irradiated with 0, 0.5, 1, 2, 4 and 5 Gy at a dose rate of 0. 488 Gy/min. MN-RET from peripheral blood was scored with FCM and MN-PCE from bone marrow was scored with manual microscopy at 24, 48 and 72 h post-irradiation. Results Both MN-RET and MN-PCE rates increased with doses in the range of 0 - 5 Gy at 24, 48 and 72 h after WBI. The dose-response relationship can be fit with linear equations （ t=10.26-25.77, P〈0.05 ）. The correlation coefficients between MN-RET from peripheral blood and MN-PCE from bone marrow were highly significant （r =0.986- 0.996, P〈 0.05）. Conclusions In view of its simplicity, accuracy and high- throughput capacity, FCM scoring of peripheral blood MN-RET may be a candidate for radiation biodosimetry. More work should be carried out on human specimens to investigate this possibility.

关 键 词：辐射 小鼠 外周血网织红细胞微核 骨髓嗜多染红细胞微核 流式细胞术 生物剂量计 

分 类 号：R144[医药卫生—公共卫生与预防医学]