安多霖对微波辐射致大鼠脑损伤的预防作用研究  

Prevention effects of AduoLa Fuzhenglin on brain injury induced by microwave radiation in rats

作　　者：任俊辉[1] 彭瑞云[1] 张静[1] 李静[1] 高亚兵[1] 王水明[1] 董霁[1] 左红艳[1] 苏镇涛[1] 周红梅[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中华放射医学与防护杂志》2011年第2期190-193,199,共5页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的探讨安多霖对微波辐射致大鼠脑损伤的预防作用。方法140只二级雄性Wistar大鼠随机分为5组：健康对照组、辐射对照组、低浓度（0.75g-g·kg^-1·d^-1）预防组、中浓度（1．5g·kg^-1·d^-1）预防组及高浓度（3g·kg^-1·d^-1）预防组。预防组每日1次灌胃给予安多霖溶液，连续给药2周。给药结束后采用30mW／cm。微波辐射大鼠15min。于处理后6h、7d和14d，采用Morris水迷宫检测大鼠学习和记忆能力，高效液相色谱检测海马氨基酸类神经递质含量，光镜和电镜观察海马组织学和超微结构变化。结果微波辐射后7d内，大鼠学习和记忆能力下降（F=0．000-0.043，P〈0.05）；微波辐射后6h，4种氨基酸类神经递质含量均降低，其中谷氨酸、甘氨酸及1-氨基丁酸降低明显（F=0.000-0.007，P〈0.01）；微波辐射后6h、7d，海马组织水肿，神经元变性；神经元线粒体肿胀、空化，内质网扩张，突触问隙模糊，血管周间隙增宽。低浓度预防组上述变化与辐射对照组相似。中浓度和高浓度预防组微波辐射后7d内，大鼠学习和记忆能力损伤不明显，两者与辐射对照组相比，差异有统计学意义（F=0.015～0.028，P〈0.05）；微波辐射后6h，4种氨基酸类神经递质含量均无明显下降，其中谷氨酸含量接近正常，两者与辐射对照组比，差异有统计学意义（F=0.000～0.042，P〈0.05）；微波辐射后6h、7d，海马组织无明显损伤。结论30mW／cm。微波辐射可引起大鼠学习和记忆能力下降、海马氨基酸类神经递质代谢紊乱及海马组织学和超微结构损伤；1．5和3g·kg^-1·d^-1安多霖对微波辐射致大鼠脑损伤有预防作用。1.5g·kg^-1·d^-1安多霖为预防微波辐射致大鼠脑损伤的有效剂量。Objective To study the prevention effects of AduoLa Fuzhenglin （ADL） on the brain injury induced by microwave radiation in rats. Methods A total of 140 male Wistar rats were divided randomly into 5 groups, including control group, microwave exposed group, low dosage （O. 75 g.kg-1.d-t） group, middle dosage （1.5g°kg^-l°d-1） group and high dosage（3 g°kg^-l°d^-1） group. Rats in three ADL groups were lavaged with ADL per day for 2 weeks before radiation. After administration, rats were exposed to microwave at 30 mW/cm2 for 15 min. The abilities of learning and memory were detected by Morris water maze, and the contents of amino acids neurotransmitter of hippocampus were detected by HPLC, then the histology and uhrastrueture of hippoeampus were observed with light and electron microscope at 6 h, 7 and 14 d after exposure. Results The abilities of learning and memory were declined （F = 0.000-0.043, P 〈0.05） from 6 h to 7 d after exposure, and the contents of four kinds of amino acid neurotransmitter in hippocampus were decreased, of which GLU, GLY and GABA were decreased significantly （F = 0.000-0.007, P 〈 O.O1 ） at 6 b after exposure, then tissue edema, neuronal degeneration, neuron mitochondria swelling and cavitation, endocytoplasmic reticulum broaden, synaptic cleft blurred, and perivascular space widen were found in the bippocampus at 6 b and 7 d after exposure. The changes in low dosage group were similar to those of the radiation group. However,in middle and high dosage groups, the abilities of learning and memory were normal to some extent with the significant differences compared to the radiation group from 6 h to 7 d after exposure（ F = 0. 015- 0.028, P 〈 0.05）. The contents of four kinds of amino acid neurotransmitter were not decreased, especially GLU contents close to normal level. There were significant differences between middle and high dosage groups and radiation group at 6 h after exposure （F = 0.000-0.042, P 〈0.05 ）. Moreover, no obvious injury in the hippoc

关 键 词：安多霖 微波 大鼠 脑损伤 预防 

分 类 号：R285.5[医药卫生—中药学]