半导体激光及CO_2激光对灰黄霉素产生菌的诱变  

The Mutation Effect of Griseofulvum Producing Strain by LD-Laser and CO_2 Laser

作　　者：张剑清[1,2] 王春梅[1,2] 毛宁[1,2] 

机构地区：[1]教育部工业微生物工程中心,福建福州350108 [2]福建师范大学生命科学学院,福建福州350108

出　　处：《应用激光》2011年第2期181-184,共4页Applied Laser

摘　　要：以灰黄霉素产生菌GM120-43为出发菌株,采用半导体激光和CO_2激光进行诱变。结果,仅在100mW低功率条件下,半导体激光诱变获得正突变菌株LD100-1,突变株LD100-1固体发酵产灰黄霉素的效价为119766u/g,与出发菌株GM120-43相比提高了33.82%,且遗传性状稳定;用CO_2激光进行诱变时,获得突变株C1448-1,其固体发酵产灰黄霉素比原始菌株提高22.58%,但是性状不稳定,在传代过程中可能发生回复突变,效价降低于出发菌株。The Griseofulvin producing strain GM120-43 was taken as the original strain, which was mutated by LD-Laser and CO2 Laser. The results indicated that the mutant strain LD100-1 was obtained just at 100 mW low power when mutated by LD-Laser. The griseofulvin produced by LD100-1 with solid-state fermentation, which titer of was 119 766 u/g and it was 33.82% higher than original strain GM120-43,the genetic stability of the high productivity was stable at the same time, The mutant strain C1448-t was obtained by CO2 Laser, which solid-state fermentation producing griseofulvin was 22.58% higher than parent strain, but the character of mutant strain C1448-1 was not stable, which may happened reversible mutation that cause the titer of this strain was lower than original strain.

关 键 词：半导体激光 CO2激光 灰黄霉素 效价 

分 类 号：Q631[生物学—生物物理学]