β2糖蛋白I与乙型肝炎表面抗原的结合作用  

Factors affecting binding of beta 2-glycoprotein I to hepatitis B surface antigen in the serum of patients with chronic hepatitis B

作　　者：何川[1] 高普均[2] 荆雪[3] 吴扬[1] 

机构地区：[1]吉林大学第一医院急诊内科,吉林省长春市130000 [2]吉林大学第一医院肝胆胰内科,吉林省长春市130000 [3]青岛大学医学院附属医院消化内科,山东省青岛市266000

出　　处：《世界华人消化杂志》2011年第9期887-891,共5页World Chinese Journal of Digestology

基　　金：吉林省科技发展计划基金资助项目;No.200705106~~

摘　　要：目的:探讨慢性乙型肝炎(CHB)患者血清与β2糖蛋白I(β2-GPI)结合的影响因素.方法:利用125I标记rHBsAg和β2-GPI,通过液相放射免疫法分别测定血浆中β2-GPI、大肠杆菌M15表达的rβ2-GPI与125I-HBsAg的结合常数(Ka).选取23例血清,其中CHB HBeAg阳性组9例,CHB HBeAg阴性组9例,正常对照组5例,测定其与125I-β2-GPI的结合率.结果:血浆β2-GPI组和rβ2-GPI组的Ka值分别为(2.795±1.846)×108L/mol、(3.001±1.049)×108L/mol.利用嵌套实验设计分析,两组来源不同的β2-GPI的结合常数(Ka1、Ka2)无统计学差异.HBeAg阳性组与阴性组的结合率具有统计学差异(33.200%±11.960%vs54.540%±9.990%,P<0.05).并发现HBeAg阳性组内的不同水平ALT的结合率有差异(42.392%±6.860%vs21.720%±1.442%,P<0.05).结论:血浆中HBsAg与β2-GPI可能有较强的亲和力,β2-GPI的糖基化结构对二者结合作用无影响.HBeAg、ALT影响HBsAg与β2-GPI的结合.AIM：To investigate factors affecting binding of beta 2-glycoprotein I（β2-GPI） to hepatitis B surface antigen（HBsAg） in the serum of patients with chronic hepatitis B（CHB）.METHODS：Recombinant HBsAg（rHBsAg） was radiolabeled with Na 125I and used to measure the affinity constant（Ka） of serum β2-GPI or recombinant β2-GPI with HBsAg.Serum samples were collected from 9 HBeAg-positive,9 HBeAg-negative CHB patients and 5 normal controls to measure the binding rate of 125I-β2GPI with serum HBsAg.RESULTS：There was no statistically significant difference between the affinity constants of serum β2-GPI and recombinant β2-GPI with HBsAg [（2.795 ± 1.846） × 108 L/mol vs（3.001 ± 1.049） ×108 L/mol].A significant difference was noted in the binding rate of I-β2-GPI with HBsAg between HBeAg-positive and-negative patients（33.200% ± 11.960% vs 54.540% ± 9.990%,P 0.05） and between HBeAg-positive patients with different ALT levels（42.392% ± 6.860% vs 21.720% ± 1.442%,P 0.05）.CONCLUSION：The binding affinity of β2GPI to serum HBsAg is strong in CHB patients,which is not affected by the glycosylation of β2GPI.HBeAg and ALT levels affect the binding of HBsAg to β2-GPI in the serum of CHB patients.

关 键 词：Β2糖蛋白I 乙型肝炎表面抗原 蛋白结合 

分 类 号：R512.62[医药卫生—内科学]