柘叶饲养对家蚕消化液中抗核多角体病毒(BmNPV)相关蛋白活性的影响  

Activities of anti-BmNPV proteins in digestive juice of the silkworm(Bombyx mori) reared on cudrang(Cudrania tricuspidata) leaves

作　　者：王晓强[1] 冯伟[1] 谢洪霞[1] 周围[1] 张冉[1] 高红[1] 万永继[1] 

机构地区：[1]西南大学生物技术学院,重庆北碚400716

出　　处：《昆虫学报》2011年第4期404-408,共5页Acta Entomologica Sinica

基　　金：公益性行业(农业)科研专项(nyhyzx07-020-01);国家现代农业产业技术体系建设专项(nycytx-27-gw203)

摘　　要：为探讨柘叶Cudrania tricuspidata饲养家蚕Bombyx mori易感染核多角体病毒(BmNPV)的机制,本实验比较研究了分别以柘叶和桑叶Morus alba饲养家蚕后其消化液中抗病毒蛋白活性的差异。结果表明:家蚕经柘叶饲养后消化液中红色荧光蛋白(red fluorescent protein,RFP)的强度无明显变化,但柘叶饲养蚕消化液中脂肪酶、胰蛋白酶的活性显著低于桑叶饲养蚕,柘叶饲养蚕消化液中脂肪酶和胰蛋白酶的活性分别为1421.71±202.60U/L和19.67±8.17U/mL,桑叶饲养蚕脂肪酶和胰蛋白酶的活性分别为1976.03±139.92U/L和199.18±181.71U/mL。这些结果说明,消化液中脂肪酶和胰蛋白酶的活性水平低与柘叶饲养蚕易感染核型多角体病毒(BmNPV)可能相关。It had been reported that silkworms Bombyx mori reared on cudrang （Cudrania tricuspidata） leaves are sensitive to B. mori nucleopolyhedrovirus （BmNPV）. In order to explore the mechanisms of this phenomenon,we investigated the activities of different anti-BmNPV proteins in digestive juice of the silkworm reared on cudrang leaves and mulberry （Morus alba） leaves,respectively. The results showed that there was no significant change in intensity of red fluorescent protein （RFP） in the digestive juice of the silkworm reared on cudrang leaves. However,the enzymatic activities of lipase and trypsin in the silkworm reared on cudrang leaves were 1 421.71±202.60 U/L and 19.67±8.17 U/mL,respectively,much lower than those of the silkworms reared on mulberry leaves,which were 1 976.03±139.92 U/L and 199.18±181.71 U/mL,respectively （P〈0.05）. The results suggest that the susceptibility of silkworms to BmNPV may be related with the lower activities of lipase and trypsin in digestive juice.

关 键 词：家蚕 柘树 家蚕核多角体病毒 红色荧光蛋白 脂肪酶 胰蛋白酶 

分 类 号：Q965.8[生物学—昆虫学]