低氧诱导因子-1α在后处理心肌保护效应中的作用  

The effects of hypoxia inducible factor-I on the cardioprotection of postconditioning against ischemia/reperfusion injury

作　　者：赵焕新[1] 李晓宇[2] 武烨[2] 王晓樑[2] 赵荣瑞[2] 刘慧荣[2] 

机构地区：[1]山西中医学院生理教研室,太原市030024 [2]山西医科大学生理教研室

出　　处：《中国心血管病研究》2011年第5期382-385,共4页Chinese Journal of Cardiovascular Research

基　　金：山西省高等学校优秀青年学术带头人支持计划

摘　　要：目的 探讨后处理减轻心肌缺血/再灌注损伤的效应与低氧诱导因子-1α（HIF-1α）的关系.方法 建立大鼠心肌缺血/再灌注及后处理模型,以心肌梗死面积、乳酸脱氢酶（LDH）活性来反映心肌的损伤程度,运用Western blot、real time-PCR等方法检测心肌组织中HIF-1α的表达情况,以探讨其是否参与了后处理的心肌保护效应.结果 后处理缩小了缺血/再灌注所致的心梗面积,降低了血清LDH活性,同时上调了心肌组织中HIF-1α的表达.预先给予HIF-1α脯氨酸羟化酶抑制剂DMOG使HIF-1α表达上调后,后处理减轻心肌损伤的效应进一步增强.结论 后处理减轻缺血/再灌注所致心肌损伤的效应可能与其上调心肌组织中HIF-1α的蛋白表达有关.Objective To explore whether HIF-1α have the effects on the cardioprotection of posteondi- tioning against ischemia/reperfusion injury. Methods Adult male Wistar rats underwent the left anterior descending coronary artery 30 min myocardial ischemia （I） and 180 min reperfusion （R） with or without postconditioning （PostC, i.e., 3 cycles of 10s-R/10s-I after onset of the reperfusion）. Another group rats were pretreated with a 40 mg/kg prolyl hydroxylase inhibitor dimethyloxalylglycine （DMOG） 24 h before PostC to stabilize HIF-1α. My- ocardial damage was evaluated by infarct size and LDH release. The expression of HIF-1α was examined by West- em blot and real time-PCR. Results Postconditioning attenuated myocardial infarct size and LDH activity compared with I/R group. Meanwhile, the expression of HIF-1α in protein level was further increased by postconditioning. After upregulation of HIF-1α by DMOG, the cardioprotective effects of postconditioning were further enhanced. Conclusion Postconditioning increased the expression of HIF-1α, which may play a role in the cardioprotective effects of postconditioning.

关 键 词：后处理 缺血/再灌注 低氧诱导因子-1 

分 类 号：Q95-33[生物学—动物学] R542.2[医药卫生—心血管疾病]