螺旋藻抗石英尘对肺泡巨噬细胞影响的实验研究  

Experimental Studies on Effects of Spirulina on Anti damage to Pulmonary Alveolar Macrophage Caused by Quartz Dust

作　　者：徐永芳 吴开国[1,2] 杨莉[1,2] 吴超伟[1,2] 袁秀玲[1,2] 

机构地区：[1]南宁市卫生防疫站 [2]广西医科大学

出　　处：《中国工业医学杂志》1999年第4期212-215,共4页Chinese Journal of Industrial Medicine

摘　　要：观察不同时点螺旋藻对染尘肺泡巨噬细胞的保护功能。方法采用肺泡巨噬细胞体外培养方法。结果随培养时间延长，各组ＬＤＨ、ＭＤＡ含量均明显升高，ＳＯＤ含量均明显下降，尤其在１２小时前变化较明显，但经ＳＰ处理的两组则有明显的拮抗作用；表现在：各孵育时点ＳｉＯ２组ＬＤＨ、ＭＤＡ含量最高，ＳＯＤ最低，其次分别为ＳｉＯ２＋ＳＰ、生理盐水组、ＳＰ组，各组ＬＤＨ、ＭＤＡ、ＳＯＤ含量经统计学处理均有显著性差异（Ｐ＜００１）。结论（１）ＳＰ具有提高染尘ＰＡＭ体外抗氧化水平和保护ＰＡＭ、增强ＰＡＭ吞噬功能作用。（２）ＳＰ提高ＰＡＭ体外抗氧化水平作用可能与其含有丰富营养成分有关。Objective To study the protective effects of spirulina on pulmonary alveolar macrophage exposed to quartz dust at different time.Methods Alveolar macrophage was cultured in vitro and its levels of LDH MDA and SOD were determined.Results Levels of LDH and MDA in the alveolar macrophage elevated and level of SOD reduced significantly with the duration of culture,especially before 12 hours for culture.But,treatment with spirulina had obviously antagonist effects on it.Content of LDH and MDA reached the highest and that of SOD the lowest in those treated with silica dust,as compared with those treated with silica and spirulina,and with normal saline or with spirulina.There was significant difference in levels of LDH,MDA and SOD between different treatments(P<0 01).Conclusion It suggested that spirulina could increase anti oxidation in vitro of alveolar macrophage exposed to silica,protect it and improve its phagocytosis,which could be attributed to its rich nutritional components.

关 键 词：螺旋藻 肺泡巨噬细胞 脂质过氧化 石英尘 

分 类 号：R135.2[医药卫生—劳动卫生]