2个猪品种的ATP2B1基因表达与ETEC F4粘附性关联分析  

Assocaition between the Expression of ATP2B1 and Enterotoxigenic Escherichia Coli F4 Adhesive Phonetype in 2 Swine Breeds

作　　者：刘定发[1] 马海明[2] 何俊[2] 蒋隽[2] 

机构地区：[1]广东省农业科学院畜牧研究所,广东广州510640 [2]湖南农业大学动物科学技术学院,湖南长沙410128

出　　处：《家畜生态学报》2011年第2期19-22,共4页Journal of Domestic Animal Ecology

基　　金：国家自然科学青年基金项目(30800607);全国优秀博士学位论文专项基金(2009027)

摘　　要：以ATP2B1基因作为仔猪腹泻相关的候选基因,在2个品种63头猪的小肠组织中,采用实时荧光定量PCR技术对肠毒素大肠杆菌(ETEC)F4受体不同黏附表型个体进行表达量的比较分析,以验证其基因功能和作为仔猪腹泻相关候选基因的可能性。结果表明:ATP2B1基因在不同黏附表型间的表达量有显著的差异,可望作为F4ab受体的相关侯选基因。As candidate genes relevant to diarrhea traits in piglets,the genetic expression of ATP2B1 was detected in 63 pigs including 2 breeds by means of real-time PCR（RT-PCR）to check the function and the possibility of related candidate gene and contrast the differences between the ETEC F4 non-adhesive and adhesive phonetypes.The result showed that there was significant difference between the non-adhesived and adhesive in two brereds,ATP2B1 might be the candidate gene for ETEC F4 receptor.

关 键 词：肠毒素大肠杆菌 ATP2B1基因 F4粘附性 表达分析 猪 

分 类 号：S811.5[农业科学—畜牧学]