氯化汞对K_(562)和HEL细胞株作用的实验研究  

Effects of mercuric chloride (HgCl_2) on K_(562) and HEL cell lines

作　　者：张鹏[1] 李明勋[1] 刘彭[1] 孙淑娟[1] 胡龙虎[1] 王树叶[1] 杨惠芬[1] 李晓霞[1] 肖或君[1] 耿洁[1] 

机构地区：[1]哈尔滨医科大学附属第一医院血液内科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》1999年第4期269-273,共5页Journal of Harbin Medical University

摘　　要：为探讨中药水银（Hg）和轻粉（HgCl）的抗肿瘤作用，以三氧化二砷（AS2O3）及阿糖胞苷（Ara－c）为对照，应用细胞形态学、流式细胞术、MTT法和药物反应曲线等方法观察3种药物对K562和HEL细胞株的作用。结果表明，HgCl2和As2O3均能诱导562细胞凋亡，而Ara－c无此作用.HgCl2可使K(562)的G0／G1增高，提示激活P53基因可能是氯化汞诱导凋亡机制之一；HgCl2和AS2O3均可显著抑制K(562)增殖；Ara－c抑制HEL增殖作用明显强于HgCl2和As2O3三者对HEL均无诱导凋亡作用，但对K(562)和HEL均有不同程度杀伤和致死作用。HgCl2和As2O3作用相似，其主要机制可能是与Hg(2+)和AS(3+)与巯基（－SH）有极高亲和力，继而与含巯基的活性基结合、降低诸多酶的活性、损害细胞膜有关。HgCl2具有抗肿瘤作用，是一种新的诱导细胞凋亡药物。To explore the effects of HgCl2 on K562 and HEL cell lines, and compared it with HgCl2 AS2O3 and Ara-c by using morphology, flow cytometry, MTT and drug-reactive curve. The results indicated that both HgCl2 and As2O3 could induce K562 cells apoptosis ,Ala-c did not have these effects; HgCl2 could increase percentage of K56, cells in G0/G1 phase. It indicated that HgCl2 induces K562 cells apoptosis probably by triggering P53 gene; As2O3 and HgCl2 inhibited the proliferation of K562 cells significantly; the proliferation of HEL cells was inhibited by Ara-c notably although it is not sensitive to As2O3 and HgCl2 .However, they all can not induce the apoptosis of HEL cell. HgCl2 is a new drug that can induce leukemia cells apoptosis.

关 键 词：氯化汞 三氧化二砷 阿糖胞苷 K_562 HEL 

分 类 号：R979.1[医药卫生—药品]