高效液相色谱法测定吡罗昔康片的含量  

Determination of Piroxicam Tablets by High Performance Liquid Chromatography

作　　者：张宏祥 刘燕 

机构地区：[1]安徽省池州市食品药品检验所,安徽池州247000

出　　处：《现代科学仪器》2011年第2期104-105,共2页Modern Scientific Instruments

摘　　要：目的探讨吡罗昔康片含量测定的方法。方法采用GL Sciences Inertsil ODS-SP(5μm,250×4.6mm)色谱柱,以乙腈-磷酸盐缓冲液(取磷酸氢二钠7.15g,加水溶解成1000mL,用20%柠檬酸溶液调节pH值为5.0)(40:60)为流动相,检测波长为358nm,柱温为35℃,流速为1.0mL.min-1。结果吡罗昔康在21.26～106.30 mg.L-1范围内进样量与峰面积呈良好线性关系(r=0.9999),平均回收率为99.68%(n=6),RSD为0.88%。结论HPLC法测定吡罗昔康片的含量,专属性强,结果准确可靠。A method determining the piroxicam tablets is explored. Methods： GL Sciences Inertsil ODSSP（51ma, 250x4.6mm）column was used. The mobile phase was acetonitrile-phosphate buffer solution （disodium hydrogen phosphate 7.15g, dissolved in water into 1000mL, adjusted with 20% citric acid solution, pH 5.0） （40：60）. The detection wavelength was 358nm.The column temperature was 35℃and the flow rate was 1.0mL-min^-1. Results： Piroxieam in the 21.26-106.30 mg·L^-1 within the sample volume and the peak area showed good linearity （r = 0.9999）.The average recovery rate was 99.68%（n=6）, and RSD was 0.88%. Conclusion： The determination of piroxicam tablets by HPLC is specific, accurate and reliable.

关 键 词：高效液相色谱法 吡罗昔康 含量测定 

分 类 号：R927.2[医药卫生—药学]