与骨髓瘤细胞共培养时对骨髓间充质细胞表达DKK1和HGF的影响  

The "cross-talk" between bone marrow derived mesenchymal stem cells(MSCs) and U266 cell line dysregulated the expression of DKK1 and HGF in MSCs

作　　者：汤郁[1] 陆化[2] 张广莲[3] 陆益龙[3] 胡慧瑾[2] 李俊霞[2] 吴雨洁[2] 王丽霞[3] 费小明[3] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京212009 [2]南京医科大学第一附属医院血液科,江苏南京210029 [3]江苏大学附属医院血液科,江苏镇江212001

出　　处：《南京医科大学学报（自然科学版）》2011年第5期612-615,619,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金(BK2008236);江苏省高校自然科学基础研究面上项目(07KJB320074)

摘　　要：目的:研究缺铁性贫血患者骨髓间充质干细胞(mesenchymal stem cells,MSCs)在与骨髓瘤细胞株U266共培养过程中,骨髓瘤细胞对MSCs DKK1和HGF表达的影响。方法:将体外培养的骨髓MSCs,在Transwell的条件下与骨髓瘤细胞株U266共培养后,检测骨髓MSCs的生长、培养液上清中肿瘤坏死因子-α(TNF-α)的水平,以及Dickkopf 1(DKK1)和肝细胞生长因子(hepatocyte growth facfor,HGF)表达的变化。结果:骨髓MSCs与U266共培养后,与对照组MSCs比较其形态和生长未见明显改变,TNF-α的水平也没有明显升高,但经Reat-time PCR检测,骨髓MSC的DKK1和HGF mRNA表达水平有改变,其中在共培养第5天时间点DKK1和HGF均增高(P<0.05),但在第8和12天2个时间点没有统计学差异(P>0.05)。结论:骨髓MSCs在与U266骨髓瘤细胞共培养后,U266诱导骨髓MSCs的HGF和DKK1表达出现异常。Objective：To ivestigate the possible effects on normal bone marrow derived mesenchymal stem cells（MSCs） when co-cultured with myeloma cell line U266.Methods：Bone marrow MSCs from normal subjects were co-cultured with U266 by transwell.On the given time point,the levels of TNF-α in the medium,the mRNA expression levels of Dickkopf 1（DKK1） and hepatocyte growth factor（HGF） were mesured.Results：No evident morphologic and proliferative alterations could be observed in bone marrow derived MSCs after co-cultured with U266,and there was also no detectable increase in the level of TNF-α in superant of the co-culture system.However,the mRNA expression levels of DKK1 and HGF were upregulated in bone marrow MSCs after 5 days’ co-culture with U266.Conlusion：The ＂cross-talk＂ between bone marrow MSCs and U266 induced dysregulation of DKK1 and HGF,which might be involved in the pathogenesis of multiple myeloma.

关 键 词：骨髓瘤 间充质干细胞 DICKKOPF 1 肝细胞生长因子 骨髓微环境 

分 类 号：R730.231[医药卫生—肿瘤]