不同代数RAW264.7细胞分化为破骨细胞样细胞的能力研究  

Comparison of stability of RAW264.7 cells at different passages to osteoclast in vitro

作　　者：潘懿[1] 张伟[1] 张克勤[1] 

机构地区：[1]南京医科大学第一附属医院内分泌科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第5期616-619,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:观察不同代数RAW264.7细胞形成破骨细胞样细胞的能力是否改变。方法:以细胞核因子κB受体激活物的配体(receptor activator of NF-κB ligand,RANKL)为诱导剂,选用第6、9、13、18、24代RAW264.7细胞在体外诱导6天,固定细胞行抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)染色,计数阳性细胞数及总细胞数。结果:第6、9、13、18、24代细胞均有TRAP染色阳性细胞;第6、9、13、18代细胞的阳性率无显著差异(P>0.05),而第24代与第6、9、13、18代细胞的阳性率显著降低(P<0.01)。结论:RANKL可以诱导RAW264.7细胞形成破骨细胞样细胞;RAW264.7细胞经多次传代后分化为破骨细胞样细胞的能力明显减弱。Objective：To determine the differentiation capability of RAW 264.7 cells following subcultures.Methods：The cells were treated with receptor activator of NF-κB ligand（RANKL）.The 6th,9th,13th,18th and 24th passages were treated with or without RANKL for 6 days.Tartrate-resistant acid phosphatase（TRAP） staining was used to observe the formation of osteoclasts.Results： RAW 264.7 cells at different passages all have TRAP-positive cells.There were no obvious differences among 6th,9th,13th and 18th in positive rate（P 〉 0.05）,and the positive rate of 24th passage were significantly lower than that of other passages.Conclusion： RANKL can induce RAW 264.7 cells to form osteoclast.If cultured for long time,the differentiation capability of this cell line was reduced.

关 键 词：RAW264.7细胞 破骨样细胞 RANKL 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]