刺五加苷B对MPP^+诱导PC12细胞损伤ERK通路的影响  

The effect of Eleutheroside B on ERK1/2 of MPP^+ -induced PC12 cells

作　　者：董杨[1] 刘树民[1,2] 安丽凤[3] 卢芳[1,2] 唐波[1] 周世慧[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,黑龙江哈尔滨150040 [2]北药基础与应用研究省部共建教育部重点实验室,黑龙江哈尔滨150040 [3]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《分子诊断与治疗杂志》2011年第3期155-158,共4页Journal of Molecular Diagnostics and Therapy

基　　金：国家科技重大专项课题(2009ZX09103);国家自然科学基金青年科学基金(30901974);黑龙江省杰出青年科学基金(JC200705);教育部"春晖计划"合作科研项目

摘　　要：目的观察刺五加苷B对MPP+诱导损伤的PC12细胞ERK1/2蛋白及相关转录因子表达的影响,探究其神经保护作用机制。方法以MPP+损伤的PC12细胞为模型组,以正常PC12细胞为对照组,Western blot法检测细胞ERK1/2表达及磷酸化水平,荧光定量PCR法检测c-fos和c-jun mRNA的表达水平。结果模型组ERK1/2磷酸化水平较对照组显著降低(P<0.01),c-fos和c-jun表达明显上调,差异有统计学意义;给予刺五加苷B(10mg·L-1)后,受损细胞ERK1/2的磷酸化水平明显升高,c-fos和c-jun基因的过表达水平降低。结论刺五加苷B对MPP+诱导的PC12细胞损伤的保护作用机制可能与提高ERK1/2蛋白磷酸化水平,避免诱发c-fos和c-jun基因过表达有关。Objective To investigate the neuroprotective mechanisms of Eleutheroside B by observing the expression of ERK1/2 and relevant transcription factors on the MPP ＋ -induced PC12 cells. Methods PC12 cells were treated with MPP ＋ to establish cellular models of Parkinson＇s disease. Western blot was performed to determine the level of phospho-ERK1/2. The expression of c-fos and c-jun can be measured by using the method of FQ-PCR. Results The level of phospho-ERK1/2 significantly decreased and the expression of c-fos and c-jun were upregulated in model group compared with the control group. Compared with model group, treatment with Eleutheroside B（10 mg·L -1 ） significantly increased the level of phospho-ERK1/2, but decreased the expression of c-fos and c-jun in damaged cells. Conclusion The neuroprotective mechanisms of Eleutheroside B may be correlated with an increase of ERK1/2 phosphorylation and down-regulation of c-fos and c-jun expression.

关 键 词：帕金森病 刺五加苷B PC12细胞 ERK1/2 1-甲基-4-苯基吡啶离子 c-fos C-JUN 

分 类 号：R285[医药卫生—中药学]