薄层色谱法鉴别加味三黄片的有效成分及薄层扫描法测定加味三黄片中小檗碱的含量  

Identification of Chief Effective Component of Jiawei Sanhuang Tablet with Thin-Layer Chromatography(TLC) and Determination of Content of Berberine in Jiawei Sanhuang Tablet with TLC Scanning

作　　者：杨建明[1] 李立恒[2] 王志勇[1] 曹亮[2] 薛贵平[1] 黄红娜[1] 

机构地区：[1]河北北方学院基础医学院药学系,河北张家口075000 [2]河北北方学院附属第一医院,河北张家口075000

出　　处：《河北北方学院学报（自然科学版）》2011年第2期31-34,39,共5页Journal of Hebei North University:Natural Science Edition

摘　　要：目的建立薄层色谱法鉴别加味三黄片中主要有效成分的方法及薄层扫描法测定三黄片中小檗碱的含量的方法.方法采用甲醇回流提取法制备供试品,用薄层色谱法鉴定三黄片中的小檗碱,黄芩苷,大黄素及大黄酚等成分,利用薄层扫描法测定加味三黄片中小檗碱的含量,扫描条件为:检测波长λ=345 nm.结果加味三黄片供试品与对照品色谱在相同位置上呈相同颜色的斑点,说明供试品中含有小檗碱,黄芩苷,大黄素,大黄酚等成分,测得供试品溶液中小檗碱的浓度为0.166 mg.mL-1.加味三黄片中小檗碱的含量为0.996mg/片.结论加味三黄片是在三黄片基础上制得,其主要成分含量经检测与三黄片相仿.采用薄层色谱法鉴定加味三黄片的主要成分操作简便,结果准确.采用薄层扫描法测定加味三黄片中小檗碱的含量结果可靠,可作为加味三黄片的质量控制标准之一.Objective To develop the methods of TLC to identify the chief effective components of Sanhuang tablet and the methods of TLC scanning to determine the content of Berberine in Sanhuang tablet.Methods The testing solution was prepared by refluxing in methanol.The Berberine,Emodin,Chrysophanol and Baicalin in Jiawei Sanhuang tablet were identified by the motheds of TLC,and the content of Berberine in Jiawei Sanhuang tablet was determined by the mothed of TLC scanning.The detection wavelength was 345 nm.Results The testing solution of Jiawei Sanhuang tablet and the reference substance both showed the same color spots in the same position by TLC.It showed that the Jiawei Sanhuang tablet contained Berberine,baicalin,emodin and Chrysophanol.The concentration of Berberine in the testing solution was 0.166 mg·mL-1.The concentration of Berberine in Jiawei Sanhuang tablet was 0.996 mg per tablet.Conclusion The Jiawei Sanhuang tablet prepared on the basis of Sanhuang tablet has the same chief components.Using TLC to identify the chief effective components of Sanhuang table is a simple,accurate and reliable method.Using TLC scanning to determinate the content of Berberine in Jiawei Sanhuang tablet is simple and reliable.The method may be used as one of the quality control standards of Jiawei Sanhuang tablet.

关 键 词：加味三黄片 成分鉴定 薄层色谱法 含量测定 薄层扫描法 

分 类 号：O658.12[理学—分析化学]