HPLC同时测定五味子中6种木脂素类成分  

Simultaneous Determination of Six Lignan Components in Schisandrae Chinensis Fructus by HPLC

作　　者：黄文倩[1,2] 李丽[1] 肖永庆[1] 张村[1] 于定荣[1] 麻印莲[1] 田国芳[1] 王云[1] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2011年第10期63-66,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30973874)

摘　　要：目的:建立同时测定五味子中6种木脂素类成分的HPLC含量测定方法。方法:采用HPLC,以甲醇-0.5%冰醋酸为流动相,梯度洗脱,检测波长254 nm,流速0.9 mL.min-1,柱温35℃,同时测定五味子药材中五味子甲素、五味子乙素、五味子丙素、五味子醇甲、五味子醇乙、五味子酯甲等6种木脂素类成分的含量。结果:不同产地五味子药材中均含有上述6种成分,且均以五味子醇甲的含量最高,五味子丙素含量最低。结论:建立的方法可以同时测定五味子药材中的6种木脂素类成分,为五味子及其炮制品的质量评价提供了更为全面的科学依据。Objective：To establish a HPLC method for the simultaneous determination of six lignan components（deoxyschizandrin,schizandrin B,schizandin C,schisandrin,schizandrol B,schisantherin A）in Schisandra chinensis.Method：The chromatographic separation was performed on an Agilent Eclipse XDB C18 Column（4.6 mm×250 mm,5 μm）at 35 ℃ with the methanol-0.5% glacial acetic acid as the mobile phrase in gradient elution.The detection wavelength was set at 254 nm and the flow rate was 0.9 mL·min-1.Result：Six lignans can be detected in different samples and the content of the schisandrin is the highest while the schizandin C is the lowest.Conclusion：The method that established by this paper can be used to determine six lignan components in Schisandrae Chinensis Fructus simultaneously,and provide a more comprehensive scientific basis for the quality evaluation of Schisandrae Chinensis Fructus and its processed products.

关 键 词：五味子 木脂素 含量测定 

分 类 号：R284.1[医药卫生—中药学]